A novel ratiometric fluorescent sensing method based on MnO₂ nanosheet for sensitive detection of alkaline...

Alkaline phosphatase (ALP) is an important biomarker for clinical diagnosis. Abnormal levels of ALP are closely related to many diseases. In this contribution, a ratiometric fluorescent sensing method based on the competition between two oxidation-reduction reactions related to MnO₂ nanosheets was developed for ALP detection. Moreover, an advanced model was derived for the quantitative analysis of the fluorescence measurements obtained by the proposed ratiometric fluorescent sensing method. With the aid of the advanced model, the proposed method achieved satisfactory quantitative results for ALP in real-world serum samples, with accuracy comparable to the corresponding results obtained by an automatic biochemical analyzer. Its recovery rates for the spiked serum samples were in the range of 98.4-115.0%, which were quite satisfactory considering the complexity of the matrices of the samples. The limit of detection and limit of quantification were estimated to be 0.09 and 0.30 U L^-1, respectively. Therefore, it is reasonable to expect that the proposed ratiometric fluorescent sensing method can be further developed to be a competitive alternative for ALP detection.