Metal organic framework assisted in situ complexation for miniaturized solid phase extraction of organic mercury in...

Zirconium-based metal-organic frameworks, namely Zr-based MOF, was employed as adsorbent material in the miniaturized solid phase extraction of organic mercury compounds in food prior to capillary electrophoresis-diode array detector analysis. The synthesized adsorbent was characterized by different spectroscopic techniques. Parameters influencing the extraction and complexation of methylmercury chloride, ethylmercury chloride and phenylmercury chloride such as type of eluent solvent, type and amount of adsorbent were investigated. In addition, linear ranges contained 2.00-300.00 ng mL^-1 for MeHg⁺, 5.00-500.00 ng mL^-1 for EtHg⁺ and PhHg⁺, and the established method presented good linearity (R² ≥ 0.998). Under the optimized experimental conditions, the ranges of detection limit and quantitation limit were 0.022-0.067 ng mL^-1 and 0.073-0.220 ng mL^-1, respectively. The relative standard deviations of intra- and inter-day analysis were less than 3.2 and 3.1%, respectively. Trueness of the present method was successfully accomplished by means of the recovery assays (81.4-98.5%) in the blank samples with two concentration levels. The repeatability %RSD of the method was lower than 2.7%. Overall, the developed approach proved to have the latent capability to be utilized in routine analysis of organic mercury compounds in fish and Dendrobium officinale.