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Published on: April 21, 2016
Capacitance electrochemical biosensor based on silicon nitride transducer for TNF-α cytokine detection in artificial
Mohamed Bahri1, Abdoullatif Baraket2, Nadia Zine2
1NANOMISENE Lab, LR16CRMN01, Centre for Research on Microelectronics and Nanotechnology of Sousse, Technopole of Sousse B.P. 334, Sahloul, 4034, Sousse, Tunisia; University of Sousse, Higher Institute of Applied Sciences and Technology of Sousse,GREENS-ISSAT, Cité Ettafala, 4003, Ibn Khaldoun Sousse, Tunisia.
Abstract:
In the present study, we have developed a capacitance electrochemical biosensor based on silicon nitride substrate (Si3N4/SiO2/Si[P]/Al) for Tumour Necrosis Factor Alpha (TNF-α) cytokines detection. Micro-contact printing, Fluorescence microscopy characterization and contact angle measurement (CAM) were carried out during the bio-functionalization of the biosensor surface. Mott-Schottky analyses were applied for TNF-α detection within the range of 1 pg/mL to 30 pg/mL in which the immunosensor has exhibited a good linearity, a sensitivity of 4 mV.pM-1 and 4.4 mV.pM-1 in PBS and artificial saliva (AS) respectively. While the LOD was found at 0.38 pg/mL and 1 pg/mL in PBS and AS respectively. The developed immunosensor has also demonstrated a high and good selectivity for TNF-α detection in human AS when compared to other interferences like Cortisol and Interleukin-10. The performances of the developed biosensor are very promising for biomedical application to predict the first sign of inflammation.
