Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Protein Complex Assembly

Protein Complex Assembly

Protein Complex Assembly

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A deep learning model captures position-specific preferences of plant regulatory sequences and suggests genes under complex regulation.

Plant physiology·2026

Same author

Bayesian inference of tissue-migration histories in metastatic cancer from cell-lineage tracing data.

Cell genomics·2026

Same author

Machine learning and multi-omic analysis reveal contrasting recombination landscape of A and C subgenomes of winter oilseed rape.

The plant genome·2026

Same author

Accessing crop genetic diversity via pangenomics.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026

Same author

Large scale quantification of natural killer cell-induced apoptosis in patient-derived organoids reveals intratumoral response heterogeneity.

NPJ precision oncology·2025

Same author

Widespread turnover of a conserved cis-regulatory code across 589 grass species.

Molecular biology and evolution·2025

Search research articles

Related Experiment Video

Updated: Dec 31, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

Legume Pangenome Construction Using an Iterative Mapping and Assembly Approach.

Haifei Hu¹, Yuxuan Yuan¹, Philipp E Bayer¹

¹School of Biological Sciences and Institute of Agriculture, University of Western Australia, Perth, WA, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|January 2, 2020

Summary

We developed a novel method to construct a legume species pangenome, enabling comprehensive analysis of genetic variations and identification of genes influencing traits. This approach leverages advanced sequencing for broader genomic insights.

Keywords:

Assembly De novo Legume Mapping Pangenome

More Related Videos

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

Related Experiment Videos

Last Updated: Dec 31, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

Area of Science:

Genomics
Plant Science
Bioinformatics

Background:

A pangenome encompasses all genomic sequences within a species, crucial for understanding genetic diversity.
Characterizing species-wide genetic variations and identifying genes linked to phenotypes is essential for biological research.
Advances in sequencing technology have made pangenome construction increasingly feasible.

Purpose of the Study:

To present a novel reference genome-based iterative mapping and assembly method.
To construct a pangenome for a legume species.

Main Methods:

Developed a reference genome-based iterative mapping strategy.
Employed an assembly method to integrate genomic sequences.
Applied the method to a legume species.

Main Results:

Successfully constructed a pangenome for the target legume species.
The method facilitates comprehensive characterization of genetic variations.
Enables mining of variable genes potentially linked to important phenotypes.

Conclusions:

The presented method is effective for legume pangenome construction.
Pangenome analysis provides a powerful tool for species-wide genomic characterization.
This approach can accelerate the discovery of genes underlying desirable traits in legumes.