Methods for Screening Legume Crops for Abiotic Stress Tolerance through Physiological and Biochemical Approaches

Legume crops are subjected to a wide range of abiotic stresses, which stimulate an array of physiological, biochemical, and molecular responses. However, different genotypes may exhibit significant variations between individual responses, which can determine their tolerance or susceptibility to these stresses. The present chapter suggests a broad range of assays that can help in understanding stress perception by plants at cellular and molecular levels. The genotypes may be sorted depending on their tolerance potential, by broadly analysing morphological, physiological, biochemical, and enzyme kinetics parameters. These assays are very beneficial in revealing the mechanism of stress perception and response in varied plant types, and have helped in discriminating contrasting genotypes. Here, we have described detailed protocols of assays which may be carried out to assess tolerance or susceptibility to abiotic stresses. The analysis, as a whole, can help researchers understand the effect of abiotic stresses on plant biochemical pathways, be it photosynthesis, redox homeostasis, metabolite perturbation, signaling, transcription, and translation. These protocols may be beneficial in identification of suitable donors for breeding programs, as well as for identifying promising candidate genes or pathways for developing stress tolerant legume crops through genetic engineering.