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Related Experiment Video

Updated: Apr 29, 2026

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

Double Rolling Circle Amplification Generates Physically Cross-Linked DNA Network for Stem Cell Fishing.

Chi Yao¹, Han Tang¹, Weijian Wu¹

¹Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology , Tianjin University , Tianjin 300350 , People's Republic of China.

Journal of the American Chemical Society

|January 2, 2020

Summary

This study introduces a novel DNA network strategy for efficiently isolating bone marrow mesenchymal stem cells (BMSCs). The method enables targeted capture, 3D cell culture, and enzyme-triggered release, preserving cell activity.

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Area of Science:

Biotechnology
Biomaterials Science
Cell Biology

Background:

Stem cell research is crucial for cell biology and therapies.
Isolating stem cells from bone marrow is challenging due to their low abundance.

Purpose of the Study:

To develop an efficient method for capturing, culturing, and releasing bone marrow mesenchymal stem cells (BMSCs).
To overcome the challenges associated with low stem cell content in bone marrow samples.

Main Methods:

A physically cross-linked DNA network was constructed using a double rolling circle amplification method.
DNA chains with aptamer sequences were used for specific BMSC anchoring.
Hybridization of DNA chains formed a 3D network for cell envelopment and separation.
Nuclease digestion was employed for enzyme-triggered cell release.

Main Results:

The DNA network strategy achieved efficient capture and 3D envelopment of BMSCs.
The physically cross-linked DNA network provided a favorable microenvironment for 3D cell culture without causing cell damage.
Enzyme-triggered release using nuclease maintained the activity of the isolated BMSCs.

Conclusions:

The developed DNA network-based strategy offers a powerful and effective method for isolating BMSCs from complex biological samples like bone marrow.
This approach facilitates stem cell separation and culture, maintaining cell viability and function.