Related Experiment Video
Updated: Dec 31, 2025
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method
Published on: October 7, 2025
A fluorescence assay for microRNA let-7a by a double-stranded DNA modified gold nanoparticle nanoprobe combined with
Yuanyuan Gao1, Jingjing Tian, Xing Zhang
1State Key Laboratory of Marine Resource Utilization in South China Sea, College of Material science and Engineering, Hainan University, Haikou 570228, China.
Abstract:
Graphene oxide (GO) is capable of quenching fluorescence in bound fuel single stranded DNA (ssDNA) labeled with dye. In the presence of target microRNA let-7a, two cascaded toehold-mediated strand displacement reactions (TSDRs) were triggered, thereby desorbing substantial π-π stacking adsorbed fuel DNA from GO through recycling amplification, simultaneously accompanied with the restoration of dye fluorescence. By coupling dsDNA-AuNPs with the GO nanosheet, the proposed strategy achieved a non-enzymatic sensitive assay with a low detection limit of 3.9 pM. Hence, the proposed approach has great potential for applications in early cancer diagnosis and clinical analysis.
