Related Experiment Video

Updated: Dec 31, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.0K

NORAD accelerates chemo-resistance of non-small-cell lung cancer via targeting at miR-129-1-3p/SOX4 axis

Qiang Huang1, Shijiang Xing2, Aiping Peng3

  • 1Department of Pharmacy, Affiliated Xiaolan Hospital of Southern Medical University, Zhongshan, China.

Bioscience Reports
|January 3, 2020
PubMed

Insights

The long non-coding RNA NORAD is upregulated in non-small cell lung cancer (NSCLC) and promotes cisplatin resistance. Targeting NORAD may restore sensitivity to chemotherapy in NSCLC patients.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in cancer chemo-resistance.
  • The specific role of NORAD in non-small cell lung cancer (NSCLC) chemo-resistance is not well understood.

Purpose of the Study:

  • To investigate the function of NORAD in cisplatin (DDP) resistance in NSCLC.
  • To elucidate the underlying molecular mechanism of NORAD's action in NSCLC chemo-resistance.

Main Methods:

  • Utilized NSCLC cell lines (H446 and A549) with induced DDP resistance.
  • Assessed NORAD expression in DDP-resistant vs. sensitive NSCLC tissues and cells.
  • Performed NORAD knockdown and miR-129-1-3p overexpression experiments.
  • Investigated the interaction between NORAD, miR-129-1-3p, and its target SOX4.

Main Results:

  • NORAD expression was significantly higher in DDP-resistant NSCLC tissues and cells.
  • High NORAD expression correlated with poor prognosis in NSCLC patients.
  • NORAD knockdown resensitized NSCLC cells to DDP.
  • NORAD sponged miR-129-1-3p, which was downregulated in DDP-resistant cells.
  • Overexpression of miR-129-1-3p reduced DDP resistance.
  • SOX4 was identified as a downstream target of miR-129-1-3p, and its overexpression reversed the effect of NORAD knockdown.

Conclusions:

  • NORAD promotes DDP resistance in NSCLC by sponging miR-129-1-3p and upregulating SOX4.
  • The NORAD/miR-129-1-3p/SOX4 axis represents a potential therapeutic target for overcoming chemo-resistance in NSCLC.
Keywords:
NORADSOX4chemo-resistancemiR-129-1-3pnon-small-cell lung cancer

More Related Videos

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.1K
Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts10:27

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

Published on: July 25, 2020

7.7K

Related Concept Videos

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.6K
Treatment Resistant Cancers02:56

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.6K
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
6.8K
Cancer Therapies02:49

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
9.7K
Combination Therapies and Personalized Medicine02:50

Combination Therapies and Personalized Medicine

Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
5.8K