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Expression and localization of DAB1 and Reelin during normal human kidney development
Anita Racetin, Marija Jurić, Natalija Filipović
1Katarina Vukojevic, Department of Anatomy, Histology and Embryology, Laboratory for Early Human Development, University of Split, School of Medicine, Šoltanska 2, 21000 Split, Croatia, katarina.vukojevic@mefst.hr.
Croatian Medical Journal
|January 3, 2020
Summary
DAB1 and Reelin proteins are present in developing human kidneys, suggesting a role in kidney formation. Their expression patterns change from fetal to postnatal stages, with high DAB1 in distal tubules and Reelin in proximal tubules during early development.
Area of Science:
- Developmental Biology
- Nephrology
- Molecular Biology
Background:
- Kidney development is a complex process involving intricate signaling pathways.
- Understanding the molecular determinants of kidney development is crucial for addressing congenital kidney diseases.
Purpose of the Study:
- To investigate the spatial and temporal expression of DAB1 and Reelin in human kidney development.
- To assess the potential role of DAB1 and Reelin as determinants of kidney structure and function.
Main Methods:
- Double immunofluorescence staining was employed on human fetal and postnatal kidney tissues.
- Antibodies against DAB1 and Reelin were used to analyze protein localization and intensity.
Main Results:
- DAB1 and Reelin exhibited distinct spatial and temporal expression patterns throughout kidney development.
- DAB1 was highly expressed in distal convoluted tubules (DCT) during fetal development, decreasing postnatally.
- Reelin showed strong expression in proximal convoluted tubules (PCT) during early fetal stages, with a significant decrease later.
Conclusions:
- The presence of DAB1 and Reelin during human kidney development suggests their involvement in organogenesis.
- High DAB1 expression in DCT indicates a potential role in tubular formation and maintenance.
- Reelin's expression profile points to a significant role in early fetal kidney development, particularly in PCT.
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