Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolomic atlas of ischemia-reperfusion injury in human lung transplants: Mitochondrial stress and primary graft dysfunction.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Microfluidic low-input profiling reveals lncRNA roles in disease.

bioRxiv : the preprint server for biology·2026
Same author

Microfluidic electroporation for drug and gene delivery: Driving innovation from single-cell precision to high-throughput preclinical and therapeutic platforms.

Advanced drug delivery reviews·2026
Same author

Early microglial priming in Alzheimer's disease revealed by ME-seq.

bioRxiv : the preprint server for biology·2026
Same author

Temporal interference stimulation for treating prolonged disorders of consciousness: A pilot study.

Brain stimulation·2026
Same author

Prenatal and postnatal effects of gestational immune activation on synaptic and neurodevelopmental pathways via epigenetic mechanisms.

Translational psychiatry·2026

Related Experiment Video

Updated: Dec 31, 2025

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

10.9K

Microfluidic Platform for Next-Generation Sequencing Library Preparation with Low-Input Samples.

Travis W Murphy1, Yuan-Pang Hsieh1, Bohan Zhu1

  • 1Department of Chemical Engineering , Virginia Tech , Blacksburg , Virginia 24061 , United States.

Analytical Chemistry
|January 3, 2020
PubMed
Summary
This summary is machine-generated.

This study introduces a microfluidic droplet system for next-generation sequencing (NGS) library preparation. The technology significantly reduces DNA input, reagent use, and manual steps, enabling faster and more accessible genomic analysis.

More Related Videos

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

18.2K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.2K

Related Experiment Videos

Last Updated: Dec 31, 2025

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

10.9K
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

18.2K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.2K

Area of Science:

  • Genomics and Molecular Biology
  • Biotechnology and Bioengineering

Background:

  • Next-generation sequencing (NGS) offers vast research potential but is limited by high DNA input requirements.
  • Current NGS library preparation methods are complex, manual, and require substantial biomaterials, hindering clinical applications.

Purpose of the Study:

  • To develop a novel microfluidic droplet-based system for efficient next-generation sequencing (NGS) library preparation.
  • To overcome the limitations of low DNA input and complex manual processes in current NGS workflows.

Main Methods:

  • A microfluidic droplet system was designed for automated NGS library preparation.
  • The system supports simultaneous preparation of 8 libraries with minimal DNA input (10 pg per library).
  • Key steps involved reducing manual pipetting and reagent consumption.

Main Results:

  • Significantly reduced the number of manual pipetting steps.
  • Decreased reagent consumption by 10-fold compared to conventional methods.
  • Enabled library preparation with extremely low DNA input (10 pg).
  • Reduced batch-to-batch variation and operator hands-on time.

Conclusions:

  • The developed microfluidic droplet system offers a semiautomated solution for low-input NGS library preparation.
  • This technology enhances the practicality of NGS for patient stratification and diagnosis by overcoming biomaterial quantity limitations.
  • The system promises to accelerate biomarker discovery and clinical applications of genomic analysis.