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Related Experiment Videos

Laser-assisted contact angle measurements.

J F Bush1, J W Huff, D L MacKeen

  • 1School of Optometry, University of Missouri-St. Louis.

American Journal of Optometry and Physiological Optics
|September 1, 1988
PubMed
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A novel laser diffraction method accurately measures contact angles on contact lenses. This technique is reproducible and correlates well with goniometry, showing mucin, but not albumin, significantly reduces contact angles.

Area of Science:

  • Optics and Materials Science
  • Surface Science and Engineering
  • Biomaterials and Medical Device Technology

Background:

  • Contact angle measurement is crucial for understanding surface wettability and biocompatibility of materials.
  • Traditional goniometry can be challenging on curved surfaces like contact lenses.
  • Developing accurate, reproducible, and convenient methods for contact angle measurement is essential in biomaterials research.

Purpose of the Study:

  • To introduce and validate a new laser diffraction technique for measuring contact angles on contact lenses.
  • To assess the influence of droplet volume, surface radius, and time on the laser-derived contact angle measurements.
  • To evaluate the effect of bovine serum albumin and bovine submaxillary mucin on the contact angles of various contact lens materials.

Main Methods:

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  • A laser beam was directed at the solid-liquid-air interface of a sessile drop on contact lenses.
  • Diffraction patterns were projected onto a screen, and the angle was measured using a protractor.
  • Measurements were taken on curved surfaces (6.5–8.9 mm radius) with varying droplet volumes (2 and 10 µL) and over time.
  • Laser-derived contact angles were correlated with goniometry measurements.
  • Contact angles were measured for different contact lens materials (Silafocon-A, Pasifocon C, PMMA) with varying concentrations of albumin and mucin.

Main Results:

  • The laser diffraction method provided contact angle measurements independent of front surface radius and droplet volume.
  • Contact angles decreased over time after drop placement.
  • Laser-derived measurements showed excellent correlation with goniometry (r = 0.86; p < 0.0001).
  • Physiological concentrations of mucin significantly decreased contact angles (8–15 degrees) on all tested materials.
  • Albumin did not significantly affect the contact angles of the tested materials.

Conclusions:

  • The laser diffraction method offers a convenient, reproducible, and accurate approach for measuring contact angles on contact lenses.
  • Surface wettability of contact lenses is significantly influenced by mucin, but not albumin, at physiological concentrations.
  • This technique has potential applications in evaluating the surface properties and biocompatibility of contact lenses and other biomaterials.