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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Dec 31, 2025

Author Spotlight: Advancing Hematopoietic Research Using Stromal Cell Isolation for Single Cell Sequencing

Author Spotlight: Advancing Hematopoietic Research Using Stromal Cell Isolation for Single Cell Sequencing

Published on: January 26, 2024

Single-cell sequencing in hematology.

Charlotte K Brierley^1,2,3, Adam J Mead^1,2,3

¹Medical Research Council (MRC) Weatherall Institute of Molecular Medicine (WIMM).

Current Opinion in Oncology

|January 3, 2020

Summary

This summary is machine-generated.

Novel single-cell technologies are revolutionizing the study of hematopoiesis and blood cancers. These methods offer unprecedented insights into cellular heterogeneity, crucial for understanding and treating myeloid malignancies.

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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Area of Science:

Hematology
Molecular Biology
Cancer Research

Background:

Bulk molecular profiling has advanced understanding of blood cancer pathogenesis and targeted therapies.
However, bulk analysis can mask intratumoral heterogeneity, impacting treatment resistance and clonal evolution.
Single-cell technologies now provide unparalleled resolution of cellular heterogeneity.

Purpose of the Study:

To review recent insights into hematopoiesis and hematological malignancies using single-cell approaches.
To focus on biological discoveries in myeloid malignancy stem/progenitor cells at single-cell resolution.
To discuss current and future clinical applications of single-cell techniques in hematological cancers.

Main Methods:

Application of novel single-cell techniques (RNA, DNA, protein expression analysis).
Analysis of stem and progenitor cells in myeloid malignancies.
Review of existing literature and emerging single-cell methodologies.

Main Results:

Single-cell resolution reveals critical aspects of intratumoral heterogeneity previously obscured by bulk analysis.
These techniques provide unprecedented insight into cellular heterogeneity in hematological malignancies.
Advancements facilitate a deeper understanding of disease mechanisms, including treatment resistance and clonal evolution.

Conclusions:

Hematology is poised to integrate single-cell techniques into routine clinical practice due to accessible liquid biopsies.
Single-cell approaches offer significant potential for improved patient management in hematological cancers.
Continued application of these technologies will drive further discoveries in blood cancer biology and therapy.