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Related Experiment Videos

Hydrocortisone secretion: production rate and pulse characterization by numerical deconvolution.

P L Toutain1, M Laurentie, A Autefage

  • 1Station de Pharmacologie-Toxicologie, Institut National de la Recherche Agronomique, Toulouse, France.

The American Journal of Physiology
|November 1, 1988
PubMed
Summary

Hydrocortisone secretion in horses is episodic. Deconvolution analysis reveals more frequent, shorter peaks compared to direct plasma analysis, offering a clearer picture of hormone release dynamics.

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Area of Science:

  • Veterinary Endocrinology
  • Pharmacokinetics
  • Hormone Secretion Dynamics

Background:

  • Hydrocortisone is secreted episodically in horses.
  • Previous analyses relied on plasma concentration profiles, potentially obscuring detailed secretion patterns.

Purpose of the Study:

  • To characterize hydrocortisone secretion peaks and troughs in horses.
  • To compare deconvolution analysis with direct plasma concentration analysis for characterizing hormone release.

Main Methods:

  • Determined kinetic parameters of hydrocortisone using intravenous bolus and perfusion.
  • Demonstrated stationarity and nonlinearity of hydrocortisone disposition.
  • Estimated 24-h hydrocortisone production rate using clearance values.
  • Reconstituted instantaneous secretory profile by deconvoluting plasma concentration data.

Related Experiment Videos

  • Analyzed secretory profile using pulse analysis program.

    • Main Results:

    • Deconvolution analysis identified significantly more peaks (17.25 +/- 1.26) with shorter durations (34.01 +/- 5.52 min) compared to direct plasma analysis (10.0 +/- 1.41 peaks, 105.25 +/- 21.24 min).
    • Total duration of secretory activity was estimated at 582.5 +/- 63.97 min via deconvolution.
    • Hydrocortisone disposition was found to be stationary and nonlinear.

    Conclusions:

    • Deconvolution analysis provides a more detailed characterization of episodic hydrocortisone secretion in horses.
    • This method offers advantages over direct plasma concentration analysis for understanding hormone release patterns.
    • The study highlights the importance of deconvolution techniques in accurately assessing endocrine secretion dynamics.