Related Experiment Video
Updated: Dec 31, 2025
17:35
A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
Published on: November 1, 2013
40.4K
Trigonelline: An alkaloid with anti-degranulation properties
Arita Dewi Nugrahini1, Momoko Ishida2, Toshifumi Nakagawa3
1Department of Applied Bioresource Science, The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime 790-8566, Japan; Department of Agro-industrial Technology, Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta 55281, Indonesia.
Molecular Immunology
|January 4, 2020
Summary
Trigonelline, found in coffee, effectively prevents allergic reactions by inhibiting mast cell degranulation. This study demonstrates its anti-degranulation properties in cellular and animal models, suggesting potential for treating allergy development.
Area of Science:
- Pharmacology
- Immunology
- Food Science
Background:
- Trigonelline is a coffee alkaloid known for nutritional and potential health benefits.
- Previous research has explored its anti-microbial, anti-carcinogenic, and anti-hyperglycemic effects.
- The anti-degranulation effect of trigonelline has not been previously investigated.
Purpose of the Study:
- To evaluate the anti-degranulation effect of trigonelline.
- To investigate the mechanisms underlying trigonelline's action on mast cells.
- To assess the potential of trigonelline in mitigating allergic responses.
Main Methods:
- In vitro studies using rat basophilic leukemia (RBL-2H3) cells to assess β-hexosaminidase release and intracellular signaling pathways.
- In vivo studies utilizing a passive cutaneous anaphylaxis (PCA) reaction in mice.
- Analysis of FcεRI-mediated intracellular signaling, including phosphorylation of PLCγ1, PI3K, and Akt.
- Investigation of trigonelline's effect on microtubule formation in RBL-2H3 cells.
Main Results:
- Trigonelline significantly suppressed antigen-induced degranulation of RBL-2H3 cells in a dose-dependent manner without cytotoxicity.
- Trigonelline inhibited FcεRI-mediated intracellular signaling pathways and the PCA response in mice.
- The compound also inhibited microtubule formation, indicating action on both calcium-dependent and independent pathways.
Conclusions:
- Trigonelline exhibits a potent anti-degranulation effect on mast cells.
- These findings suggest trigonelline can attenuate IgE-sensitized mast cell degranulation.
- Trigonelline shows promise as a therapeutic agent for preventing or treating allergic diseases.
Related Concept Videos
01:26Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin
1.0K
Directly acting muscle relaxants like dantrolene and botulinum toxin (BoNT) have distinct mechanisms and applications. Dantrolene, a hydantoin derivative, acts on the ryanodine receptor (RYR1) in skeletal muscle cells. RYR1 are calcium channels present at the sarcoplasmic reticulum membrane. In response to excitation, they release calcium ions from the sarcoplasmic reticulum to the cytosol. Calcium promotes actin-myosin-mediated contraction of muscles.
The binding of dantrolene to the RYR1...
The binding of dantrolene to the RYR1...
1.0K
01:10Drugs that Destabilize Microtubules
3.5K
Microtubules are dynamic structures and can be regulated by microtubule targeting agents (MTAs). Microtubule destabilizing drugs are a class of MTAs that destabilize and prevent microtubules' polymerization. Both natural and synthetic chemicals can be found under this class of drugs. Vincristine and vinblastine, two vinca alkaloids, and colchicine were among the first to be discovered. These drugs can affect cells in various ways, either by inducing a change in cell morphology, preventing...
3.5K
01:11Direct-Acting Cholinergic Agonists: Therapeutic Uses
1.5K
Direct-acting cholinergic agonists have many therapeutic uses in various medical fields. Choline esters, including acetylcholine, have limited clinical utility due to their non-selectivity and short duration of action. Still, acetylcholine and carbachol are applied topically during ophthalmologic surgery to induce miosis. Pilocarpine, a muscarinic and ganglionic stimulator, effectively treats open-angle glaucoma and alleviates xerostomia and dry mouth caused by radiotherapy or Sjögren...
1.5K
01:22Direct-Acting Cholinergic Agonists: Chemistry and Structure-Activity Relationship
1.9K
Cholinergic agonists or cholinomimetics mimic the action of acetylcholine to stimulate the parasympathetic nervous system. They are categorized into direct-acting and indirect-acting agents. The direct-acting cholinergic drugs induce the parasympathetic response by directly binding to the muscarinic or nicotine receptors. In comparison, the indirect-acting cholinergic drugs prevent acetylcholine hydrolysis, indirectly contributing to the extended parasympathetic response.
The direct-acting...
The direct-acting...
1.9K
01:15Drugs that Stabilize Microtubules
2.5K
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.5K
01:23Drugs Acting on Autonomic Ganglia: Stimulants
2.0K
Ganglionic stimulants activate NM nicotinic receptors in autonomic ganglia, falling into two categories: nicotine mimetics [e.g., lobeline, dimethylpiperazine, tetramethylammonium] and muscarinic receptor agonists [e.g., muscarine, methacholine]. The first category's action is rapid and blocked by nicotinic receptor antagonists, while the second category's action is delayed and blocked by atropine-like agents. Nicotine, an alkaloid, affects the heart rate by stimulating...
2.0K