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Published on: September 5, 2013
Hybrid Assembly from a Pathogenic Methicillin- and Multidrug-Resistant Staphylococcus pseudintermedius Strain
Joaquim Viñes1,2, Anna Cuscó2, Olga Francino1
1Servei Veterinari de Genètica Molecular (SVGM), Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, Barcelona, Spain joaquim.vines@vetgenomics.com olga.francino@uab.cat.
Abstract:
Here we report the genome assembly, using a hybrid approach with Illumina and Nanopore sequencing, of a pathogenic Staphylococcus pseudintermedius strain isolated from a case of canine otitis. Genome assembly confirmed the antimicrobial resistance profile (disk diffusion testing) with specific genes and mutations.
Insights
We sequenced the genome of a dog pathogen, Staphylococcus pseudintermedius, causing ear infections. This analysis identified specific genes and mutations linked to its antibiotic resistance.
Area of Science:
- Veterinary Microbiology
- Genomics
- Antimicrobial Resistance
Background:
- Staphylococcus pseudintermedius is a significant pathogen in canine infections, particularly otitis externa.
- Understanding the genetic basis of antimicrobial resistance in S. pseudintermedius is crucial for effective treatment strategies.
Purpose of the Study:
- To perform a hybrid genome assembly of a pathogenic Staphylococcus pseudintermedius strain from a canine otitis case.
- To correlate the assembled genome with observed antimicrobial resistance profiles.
Main Methods:
- Hybrid genome sequencing utilizing both Illumina and Nanopore technologies.
- Bioinformatic analysis for genome assembly and gene identification.
- Disk diffusion testing to determine antimicrobial susceptibility.
Main Results:
- Successful hybrid genome assembly of the Staphylococcus pseudintermedius strain.
- Identification of specific antimicrobial resistance genes and mutations within the genome.
- Confirmation of the strain's resistance profile through genomic data.
Conclusions:
- The genome assembly provides a valuable resource for studying Staphylococcus pseudintermedius pathogenesis and evolution.
- Genomic insights can predict and explain observed antimicrobial resistance patterns in canine pathogens.
- This study highlights the importance of integrating genomic data with phenotypic testing for comprehensive antimicrobial resistance surveillance.
