Complete Genome Sequence of Serratia Phage Muldoon

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Soren Campbell1, Cameron Atkison1, Russell Moreland1

  • 1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|January 4, 2020
PubMed

Insights

Researchers isolated and annotated Muldoon, a novel myophage (bacterial virus) targeting Serratia marcescens. This phage, related to T4-like viruses, offers insights into bacterial infection mechanisms.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Serratia marcescens is a Gram-negative bacterium associated with opportunistic infections.
  • Understanding bacteriophages (phages) is crucial for combating bacterial pathogens.

Purpose of the Study:

  • To isolate and characterize a novel phage infecting Serratia marcescens.
  • To annotate the genome of the isolated phage for further study.

Main Methods:

  • Bacterial isolation and phage identification.
  • Genomic DNA extraction and sequencing.
  • Bioinformatic analysis for gene prediction and annotation.

Main Results:

  • Isolation and naming of the Serratia marcescens myophage, Muldoon.
  • Muldoon phage genome contains 257 predicted protein-coding genes and 4 tRNA genes.
  • Phylogenetic analysis indicates Muldoon is related to T4-like phages.

Conclusions:

  • The characterization of Muldoon provides a new genetic resource for studying Serratia marcescens.
  • This myophage could have potential applications in phage therapy against S. marcescens infections.

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