Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

62.1K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.1K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

14.2K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
14.2K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

4.2K
4.2K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.7K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.7K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.2K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.2K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.4K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

CellĀ·2026
Same author

Attenuation of ATM signaling by ROS delays replicative senescence at physiological oxygen.

Molecular cellĀ·2025
Same author

Structure of the TXNL1-bound proteasome.

Nature structural & molecular biologyĀ·2025
Same author

How Shelterin Orchestrates the Replication and Protection of Telomeres.

Cold Spring Harbor perspectives in biologyĀ·2025
Same author

Replicative senescence is ATM driven, reversible, and accelerated by hyperactivation of ATM at normoxia.

bioRxiv : the preprint server for biologyĀ·2024
Same author

POT1 recruits and regulates CST-PolĪ±/primase at human telomeres.

CellĀ·2024

Related Experiment Video

Updated: Dec 31, 2025

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

14.5K

53BP1: a DSB escort.

Zachary Mirman1, Titia de Lange1

  • 1Laboratory for Cell Biology and Genetics, The Rockefeller University, New York, New York 10065, USA.

Genes & Development
|January 4, 2020
PubMed
Summary

53BP1 protein prevents harmful DNA repair outcomes by controlling DNA end processing and double-strand break (DSB) dynamics. This perspective explains its role in cancer therapy and immune system functions.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • 53BP1 is a DNA damage response factor crucial for PARP inhibitor efficacy in BRCA1-deficient cancers.
  • Recent research highlights 53BP1 as a key regulator of double-strand break (DSB) repair pathway choice.

Purpose of the Study:

  • To propose an alternative view of 53BP1's function, focusing on preventing mutagenic repair outcomes.
  • To explain the consequences of 53BP1 deficiency and its coevolution with immune system processes.

Main Methods:

  • Literature review and synthesis of existing research on 53BP1.
  • Analysis of 53BP1's role in DNA end processing and DSB dynamics.
  • Exploration of 53BP1's evolutionary relationship with class switch recombination (CSR).
Keywords:
53BP1BRCA1CSRCSTDNAPARP1PARPiShieldindouble-strand breaktelomere

More Related Videos

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.9K
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

10.6K

Related Experiment Videos

Last Updated: Dec 31, 2025

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

14.5K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.9K
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

10.6K

Main Results:

  • 53BP1 controls DNA end processing and DSB dynamics to avoid mutagenic repair.
  • 53BP1 deficiency impacts PARPi efficacy and telomere repair.
  • Proposed coevolution of 53BP1 fidelity functions with CSR in the immune system.

Conclusions:

  • 53BP1 acts as a 'DSB escort,' preventing illegitimate and potentially tumorigenic recombination.
  • This revised understanding offers new insights into DNA repair mechanisms and cancer treatment strategies.
  • 53BP1's role extends beyond pathway choice to safeguarding genomic integrity.