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Related Experiment Video

Updated: Dec 31, 2025

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
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Tissue clearing and its applications in neuroscience.

Hiroki R Ueda1,2, Ali Ertürk3,4,5, Kwanghun Chung6,7,8,9,10,11,12

  • 1Department of Systems Pharmacology, University of Tokyo, Tokyo, Japan. uedah-tky@umin.ac.jp.

Nature Reviews. Neuroscience
|January 4, 2020
PubMed
Summary

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Tissue clearing techniques enable detailed subcellular imaging of whole organs and mammals. These advanced methods accelerate research, reduce costs, and offer new insights into complex biological systems.

Area of Science:

  • Biological Imaging
  • Histology
  • Biotechnology

Background:

  • Conventional histology is time-consuming and offers limited depth penetration.
  • Existing tissue clearing methods enable subcellular resolution in intact tissues.
  • Advancements in microscopy and analysis are crucial for biological research.

Purpose of the Study:

  • To review state-of-the-art tissue-clearing methods.
  • To highlight the integration of clearing with advanced imaging and analysis.
  • To discuss the potential of tissue clearing in neuroscience and human specimen analysis.

Main Methods:

  • Tissue clearing techniques for optical access.
  • Light-sheet microscopy for imaging.
  • Automated image analysis for data processing.

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Last Updated: Dec 31, 2025

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  • Whole-organ antibody labeling for thick tissues.

    • Main Results:

    • Tissue clearing provides subcellular-level optical access to intact tissues.
    • Combined methods accelerate and reduce the cost of histology.
    • Enables whole-organ antibody labeling in thick human tissues.
    • Facilitates rapid extraction of cellular and subcellular information from complex specimens.

    Conclusions:

    • Tissue clearing offers an unbiased, system-level view of biological structures.
    • Accelerated data generation necessitates efficient computational approaches.
    • Significant future opportunities exist for tissue clearing in human neuroscience.