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Stability of histamine dihydrochloride in solution
N H Nielsen1, F Madsen, L Frølund
1Medical Dept. TTA, State University Hospital, Copenhagen, Denmark.
Allergy
|August 1, 1988
Summary
Lower concentrations of histamine dihydrochloride (HC) lose activity rapidly when stored at room temperature. For optimal bronchial challenge results, use HC dilutions within one week of opening if stored at 20°C.
Area of Science:
- Pulmonary Medicine
- Pharmacology
- Microbiology
Background:
- Histamine dihydrochloride (HC) is a key bronchoconstrictor for bronchial challenge testing.
- Understanding the stability and contamination of HC dilutions during storage is crucial for accurate diagnostic procedures.
Purpose of the Study:
- To evaluate the impact of storage temperature and duration on the activity and microbial contamination of histamine dihydrochloride (HC) dilutions.
- To provide guidelines for the optimal storage and usage of HC dilutions.
Main Methods:
- HC dilutions were stored at 20°C, 4°C, and -18°C.
- Activity, bacterial, and fungal contamination were assessed at various time points.
- Microbial analysis was performed using a specified method.
Main Results:
- HC dilutions ≤ 0.25 mg/ml showed significantly reduced activity after 1 month at 20°C, necessitating use within 1 week.
- HC dilutions stored at 4°C or -18°C remained stable for at least 6 months.
- Bacterial contamination was detected in HC dilutions < 0.5 mg/ml after 3 months of storage, irrespective of temperature. No fungal contamination was observed.
Conclusions:
- HC dilutions at concentrations of 0.25 mg/ml and below should be used within 1 week of opening and not stored for over 1 month at room temperature.
- Refrigerated or frozen storage (4°C or -18°C) ensures the stability of HC dilutions for up to 6 months.
- Careful consideration of storage conditions and concentration is necessary to maintain HC activity and prevent microbial contamination in bronchial challenge testing.