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Related Experiment Videos

Fosfomycin and plasmidic resistance.

F Baquero, M López-Brea, A Valls

    Chemotherapy
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Researchers investigated fosfomycin resistance in gram-negative bacilli. Conjugation experiments could not confirm plasmid-mediated fosfomycin resistance due to high mutation rates in the recipient strain.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Fosfomycin is a crucial antibiotic for treating Gram-negative bacterial infections.
    • The emergence of antibiotic resistance, including to fosfomycin, poses a significant public health threat.
    • Understanding the mechanisms of fosfomycin resistance, particularly plasmid-mediated transfer, is essential for effective treatment strategies.

    Purpose of the Study:

    • To investigate the potential for plasmid-mediated transfer of fosfomycin resistance among Gram-negative bacilli.
    • To determine if fosfomycin resistance can be co-transferred with other antimicrobial resistances via plasmids.
    • To identify challenges in detecting plasmidic fosfomycin resistance.

    Main Methods:

    • Conjugation experiments were performed using 60 fosfomycin-resistant Gram-negative bacilli strains.

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  • Escherichia coli K12 (nalidixic acid resistant) served as the recipient strain.
  • Selection of transconjugants was done on plates containing nalidixic acid and fosfomycin.

    • Main Results:

    • The number of fosfomycin-resistant transconjugant colonies did not exceed the spontaneous mutation rate of the recipient strain.
    • Plasmid-mediated transfer of resistance to other antimicrobials occurred in 65% of experiments.
    • Fosfomycin resistance was not co-transferred with other plasmid-mediated resistances.

    Conclusions:

    • The high spontaneous mutation rate for fosfomycin resistance in the recipient strain complicates the detection of plasmid-mediated transfer.
    • Under the tested conditions, the plasmidic nature of fosfomycin resistance could not be confirmed.
    • Further studies with modified methodologies may be needed to elucidate the mechanisms of fosfomycin resistance transfer.