Related Experiment Video

Updated: Dec 31, 2025

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
10:50

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Published on: March 14, 2019

8.6K

Cullin 4-DCAF Proteins in Tumorigenesis

Zhuan Zhou1,2, Xinxin Song1,2, Cindy M Wavelet1,2

  • 1Department of Obstetrics and Gynecology and Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.

Advances in Experimental Medicine and Biology
|January 4, 2020
PubMed

Insights

Cullin-RING ligase 4 (CRL4) is crucial for cell processes and cancer. This review details CRL4

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Cullin-RING ligase 4 (CRL4) is a key E3 ubiquitin ligase.
  • CRL4 regulates critical cellular processes and pathophysiological events.
  • CRL4's role in development and cancer etiology is increasingly recognized.

Purpose of the Study:

  • To summarize the versatile roles of CRL4 E3 ligase complexes in tumorigenesis.
  • To elucidate the in-depth mechanism of CRL4 in ubiquitination catalysis.
  • To highlight evidence from mouse models and biochemical studies.

Main Methods:

  • Review of mouse genetics studies (knockout and transgenic models).
  • Analysis of clinical data.
  • Biochemical and pathological investigations.

Main Results:

  • CRL4 complexes play diverse roles in cancer development.
  • Evidence from various study types supports CRL4's involvement in tumorigenesis.
  • CRL4's ubiquitination catalysis mechanism is central to its function.

Conclusions:

  • CRL4 E3 ligase complexes are critical regulators in cancer.
  • Understanding CRL4 mechanisms provides insights into cancer etiology.
  • Further research on CRL4 is essential for cancer therapeutics.
Keywords:
CRL4 ligase and tumorigenesisCancer etiologyCullin 4DCAFMouse models

More Related Videos

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.6K
Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells09:51

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells

Published on: June 30, 2023

1.3K

Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.5K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.3K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
6.2K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
6.4K
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
133.4K