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Updated: Dec 31, 2025

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
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Published on: July 8, 2012

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Hematopoietic Stem and Progenitor Cells (HSPCs)

Kamila Bujko1, Magda Kucia1, Janina Ratajczak1

  • 1Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA.

Advances in Experimental Medicine and Biology
|January 4, 2020
PubMed

Hematopoietic stem/progenitor cells (HSPCs) isolated from bone marrow have been successfully employed for 50 years in hematological transplantations. Currently, these cells are more frequently isolated from mobilized peripheral blood or umbilical cord blood. In this chapter, we overview several topics related to these cells including their phenotype, methods for isolation, and in vitro and in vivo assays to evaluate their proliferative potential. The successful clinical application of HSPCs is widely understood to have helped establish the rationale for the development of stem cell therapies and regenerative medicine.

Keywords:
Expansion of hematopoietic stem cellsHematopoiesisHematopoietic progenitorsHematopoietic stem cell markersHematopoietic stem cellsIn vitro assaysIn vivo assaysPrimordial germ cellsStem cell purificationVery small embryonic-like stem cells

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