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Related Concept Videos

Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Hematopoiesis01:21

Hematopoiesis

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The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
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Production of Formed Elements01:34

Production of Formed Elements

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Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
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Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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Overview of Hematopoiesis01:20

Overview of Hematopoiesis

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Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
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Lineage Commitment01:21

Lineage Commitment

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Commitment is the  process whereby stem cells:
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Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
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Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

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Hematopoietic Stem and Progenitor Cells (HSPCs).

Kamila Bujko1, Magda Kucia1, Janina Ratajczak1

  • 1Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA.

Advances in Experimental Medicine and Biology
|January 4, 2020
PubMed
Summary

Hematopoietic stem/progenitor cells (HSPCs) from bone marrow, peripheral blood, and cord blood are crucial for transplantation. This chapter details their characteristics, isolation, and evaluation for regenerative medicine applications.

Keywords:
Expansion of hematopoietic stem cellsHematopoiesisHematopoietic progenitorsHematopoietic stem cell markersHematopoietic stem cellsIn vitro assaysIn vivo assaysPrimordial germ cellsStem cell purificationVery small embryonic-like stem cells

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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
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Area of Science:

  • Hematology
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Hematopoietic stem/progenitor cells (HSPCs) have been used in transplants for 50 years.
  • Current isolation methods favor mobilized peripheral blood and umbilical cord blood over bone marrow.
  • HSPCs are foundational to stem cell therapies and regenerative medicine.

Purpose of the Study:

  • To provide an overview of Hematopoietic stem/progenitor cells (HSPCs).
  • To discuss the phenotype, isolation methods, and assays for evaluating HSPC proliferative potential.
  • To highlight the role of HSPCs in advancing stem cell therapies and regenerative medicine.

Main Methods:

  • Review of existing literature on Hematopoietic stem/progenitor cells (HSPCs).
  • Description of cell isolation techniques.
  • Explanation of in vitro and in vivo assays for assessing cell proliferation.

Main Results:

  • Hematopoietic stem/progenitor cells (HSPCs) can be isolated from various sources like bone marrow, peripheral blood, and cord blood.
  • Established methods exist for characterizing and evaluating the proliferative capacity of HSPCs.
  • The clinical success of HSPCs underpins the development of modern regenerative medicine.

Conclusions:

  • Hematopoietic stem/progenitor cells (HSPCs) are versatile and essential for transplantation and regenerative medicine.
  • Understanding HSPC biology, isolation, and function is critical for therapeutic applications.
  • The field of stem cell therapy continues to evolve, building upon the success of HSPC applications.