Related Experiment Video
Updated: Dec 31, 2025
Using Single Sensillum Recording to Detect Olfactory Neuron Responses of Bed Bugs to Semiochemicals
Published on: January 18, 2016
Analysis of the antennal transcriptome and odorant-binding protein expression profiles of the parasitoid wasp
Yanyan He1, Ke Wang1, Yang Zeng1
1Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Haidian District, Beijing 100081, China.
Abstract:
The parasitoid of whiteflies Encarsia formosa has been widely applied to reduce whitefly-mediated damage on vegetables and ornamental plants grown in greenhouses. Although its chemosensory behavior has been described, the mechanism by which E. formosa recognizes chemical volatiles at the molecular level remains unknown. In this study, we obtained 66,632 unigenes from antennae transcriptomic architecture of E. formosa, of which 19,473 (29.2%) were functionally annotated. All that matters is that we manually identified 39 odorant-binding proteins (OBPs) from above dataset, and further investigated the tissue and stage-specific expression profiles of all identified OBP genes by real-time quantitative PCR. Among these OBP genes, 32 were enriched in antennae, and 2 in body. In addition, 4 OBPs were highly expressed in pupae, and 32 in 6-hour-age adults after eclosion. In addition to identifying OBP genes from E. formosa, this study provides a molecular basis for further functional studies of OBPs and the interactions of hosts and parasitic wasps.
