Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of reducing fever01:22

Methods of reducing fever

1.2K
The signs and symptoms of fever include hot and dry skin, flushed face, thirst, muscle aches, anorexia, headache, tachycardia, tachypnea, and fatigue. Elevated body temperature is reduced using two methods: pharmacological and nonpharmacological. Proper identification and treatment of the root cause of a fever is of utmost importance.
Pharmacological Methods of Reducing Fever:
1.2K
Increased Body Temperature01:25

Increased Body Temperature

6.3K
A body temperature above  38°C  (100.4 °F) is known as fever or pyrexia, and a person with fever is termed 'febrile.' Typically, the hypothalamus, a part of the brain that acts as the body's thermostat, regulates body temperature through a thermoregulatory setpoint. It receives signals from cold and warm thermal receptors throughout the body and adjusts the body's temperature accordingly. Fever occurs when this hypothalamic setpoint is altered, usually in...
6.3K
Decreased Body Temperature01:29

Decreased Body Temperature

920
A decreased body temperature can occur in patients with hypothermia and frostbite. Heat loss with extended cold exposure overpowers the body's ability to create heat, resulting in hypothermia. Core temperature readings help classify hypothermia. Mild hypothermia is temperatures between 32 °C (89.6 °F) and 35°C (95 °F) and is caused by impaired thermoregulation. Moderate hypothermia is temperatures between 28 C (82.4 °F) and 32 °C (89.6 °F) caused by...
920
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.1K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of polyclonal and monoclonal antibody to the influenza virus nucleoprotein in different oligomeric states.

Virus research·2025
Same author

Incorporating an Afrocentric Approach into the Criminal Justice System: The Voices of Men Who Attend the Habilitation Empowerment Accountability Therapy (HEAT) Program.

Journal of evidence-based social work (2019)·2025
Same author

Analysis of polyclonal and monoclonal antibody to the influenza virus nucleoprotein in different oligomeric states.

bioRxiv : the preprint server for biology·2024
Same author

Structure-guided assembly of an influenza spike nanobicelle vaccine provides pan H1 intranasal protection.

bioRxiv : the preprint server for biology·2024
Same author

Corrigendum: Impact of adjuvant: trivalent vaccine with quadrivalent-like protection against heterologous Yamagata-lineage influenza B virus.

Frontiers in immunology·2023
Same author

Designed nanoparticles elicit cross-reactive antibody responses to conserved influenza virus hemagglutinin stem epitopes.

PLoS pathogens·2023
Same journal

Correction: Characterization of Mast2 kinase defines structural features, regulation, and substrates.

The Journal of biological chemistry·2026
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
Same journal

Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

The Journal of biological chemistry·2026
Same journal

Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

The Journal of biological chemistry·2026
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
See all related articles

Related Experiment Video

Updated: Dec 31, 2025

Short-Duration Hypothermia Induction in Rats using Models for Studies examining Clinical Relevance and Mechanisms
05:00

Short-Duration Hypothermia Induction in Rats using Models for Studies examining Clinical Relevance and Mechanisms

Published on: March 3, 2021

3.2K

Cryo-EM cools down swine fever.

John R Gallagher1, Audray K Harris1

  • 1Laboratory of Infectious Diseases, NIAID, National Institutes of Health, Bethesda, Maryland 20892.

The Journal of Biological Chemistry
|January 5, 2020
PubMed
Summary
This summary is machine-generated.

African swine fever virus (ASFV) is a complex DNA virus causing devastating global outbreaks. New cryo-EM structures reveal the ASFV virion and key proteins, aiding vaccine development.

More Related Videos

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen
06:42

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen

Published on: April 6, 2017

11.9K
Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
11:10

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

Published on: August 5, 2011

31.2K

Related Experiment Videos

Last Updated: Dec 31, 2025

Short-Duration Hypothermia Induction in Rats using Models for Studies examining Clinical Relevance and Mechanisms
05:00

Short-Duration Hypothermia Induction in Rats using Models for Studies examining Clinical Relevance and Mechanisms

Published on: March 3, 2021

3.2K
A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen
06:42

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen

Published on: April 6, 2017

11.9K
Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
11:10

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

Published on: August 5, 2011

31.2K

Area of Science:

  • Virology
  • Structural Biology
  • Immunology

Background:

  • African swine fever virus (ASFV) is a large, complex DNA virus responsible for significant global swine mortality.
  • Current ASFV outbreaks pose a severe threat to the global swine industry, with no effective vaccine available.

Purpose of the Study:

  • To present the cryo-electron microscopy (cryo-EM) structures of the complete ASFV virion.
  • To resolve the structure of the major outer-capsid protein p72 at high resolution.
  • To advance the understanding of ASFV structure-function relationships for vaccine development.

Main Methods:

  • Cryo-electron microscopy (cryo-EM) was utilized to determine the structure of the ASFV virion.
  • High-resolution analysis focused on the major outer-capsid protein p72.

Main Results:

  • The multi-layered structure of the complete ASFV virion has been elucidated.
  • The major outer-capsid protein p72 has been resolved to a higher resolution than previously achieved.
  • These structural insights provide a detailed view of the ASFV particle.

Conclusions:

  • The reported ASFV structures offer a deeper understanding of large virus architecture.
  • These findings are crucial for guiding the rational design of ASFV vaccines.
  • Further research into ASFV structural biology is expected to accelerate vaccine development efforts.