Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Synthetic Biology02:55

Synthetic Biology

5.4K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineered Modular Polyketide Synthases Elucidate How Enoylreductases Collaborate with Downstream Acyl Carrier Proteins and Ketosynthases to Set Stereocenters.

Journal of the American Chemical Society·2026
Same author

Sensitivity Analysis for Unmeasured Confounding in Causal Mediation Analysis With Survival Outcome.

Statistics in medicine·2026
Same author

Validation of a quantitative analysis method for tryptophan metabolites in asthmatic mice and bacteria using GC-MS/MS.

Journal of pharmaceutical and biomedical analysis·2026
Same author

Advanced Bayesian kernel machine regression for large-scale exposome studies: Making the impossible possible.

Innovation (Cambridge (Mass.))·2026
Same author

Skeletal editing via multi-step engineering of a modular polyketide synthase.

Nature communications·2026
Same author

The triglyceride-glucose index for diabetic retinopathy prediction in Chinese patients with type 2 diabetes: a preliminary cross-sectional study.

Frontiers in endocrinology·2026

Related Experiment Video

Updated: Dec 31, 2025

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
09:08

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

17.7K

An in vitro platform for engineering and harnessing modular polyketide synthases.

Takeshi Miyazawa1, Melissa Hirsch2, Zhicheng Zhang2

  • 1Department of Molecular Biosciences, The University of Texas at Austin, 100 E. 24th St., Austin, TX, 78712, USA.

Nature Communications
|January 5, 2020
PubMed
Summary

Researchers developed a new platform to study modular polyketide synthases (PKSs) by reconstituting the venemycin PKS. This method enables efficient production and engineering of polyketide compounds.

More Related Videos

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

10.3K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

14.7K

Related Experiment Videos

Last Updated: Dec 31, 2025

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
09:08

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

17.7K
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

10.3K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

14.7K

Area of Science:

  • Biochemistry
  • Synthetic Biology
  • Enzymology

Background:

  • Modular polyketide synthases (PKSs) are complex enzymatic machinery responsible for producing a vast array of natural products.
  • Understanding the biochemistry of PKSs is crucial for harnessing their synthetic potential.
  • A robust in vitro platform for studying these large enzyme complexes has been lacking.

Purpose of the Study:

  • To establish a functional in vitro platform for studying modular polyketide synthases (PKSs).
  • To reconstitute and characterize the venemycin PKS assembly line.
  • To explore engineering strategies for PKS modules to generate novel polyketide products.

Main Methods:

  • In vitro reconstitution of the venemycin PKS using purified polypeptides (VemG and VemH).
  • Utilizing essential substrates and cofactors including 3,5-dihydroxybenzoic acid, ATP, malonate, coenzyme A, and malonyl-CoA ligase (MatB).
  • Employing High-Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR) for product analysis and quantification.
  • Engineering PKS modules based on updated boundary definitions and testing performance against traditional boundaries.

Main Results:

  • Successful in vitro reconstitution of the venemycin PKS, producing an aromatic polyketide.
  • Achieved multi-milligram scale isolation of venemycin without chromatography, with enzyme recyclability.
  • Demonstrated superior performance of engineered synthases using updated module boundaries compared to traditional ones.
  • Generated a combinatorial library of six distinct polyketide products by varying enzymes and starter units.

Conclusions:

  • The developed platform provides a robust system for studying modular PKS biochemistry.
  • Enzyme engineering using updated module boundaries significantly enhances PKS efficiency.
  • This system facilitates the production of diverse polyketide compounds through combinatorial approaches.