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Rapid determination of amphotericin B levels in serum by high-performance liquid chromatography without interference
H Hosotsubo1, J Takezawa, N Taenaka
1Central Laboratory for Clinical Investigation, Osaka University Hospital, Japan.
Antimicrobial Agents and Chemotherapy
|July 1, 1988
Abstract:
A high-performance liquid chromatographic method for the determination of amphotericin B concentrations in human serum without bilirubin interference was developed and compared with a microbiological assay. The high-performance liquid chromatographic assay utilized a reversed-phase trimethyl silica column, simple sample preparation, and visible detection. Reproducibility studies yielded coefficient-of-variation ranges from 1.02 to 2.11% for within-day precision and 2.88 to 4.32% for between-day precision. The correlation coefficient with the microbiological assay was 0.984 for amphotericin B.