Biosynthesis of selenium nanoparticles and their effect on changes in urinary nanocrystallites in calcium oxalate...

Plant bio constituents have the ability to prepare nanoparticles, and usually, plant polyphenols are tested to reduce sodium selenite to selenium nanoparticles (SeNPs). In this work, we showed the biosynthesis of SeNPs using Ocimum tenuiflorum leaf extract. The as obtained SeNPs were in the size range of 15-20 nm and spherical in shape. Also, TEM microscopic images represented the aggregation of crystal structures as extracellular deposits. Moreover, scanning electron microscopy was performed to examine the chemical transition of calcium oxalate (CaC₂O₄) crystal's shape and structure due to the influence of SeNPs. SeNPs inhibited the aggregation and growth of CaC₂O₄ monohydrate crystals and hence the prepared SeNPs could have important prospects in medical and pharmaceutical applications as a potential inhibitor of CaC₂O₄ urinary stones.