Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.4K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.4K
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

1.6K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.6K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.5K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.5K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

633
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
633
Spin–Spin Coupling Constant: Overview01:08

Spin–Spin Coupling Constant: Overview

1.4K
In bromoethane, the three methyl protons are coupled to the two methylene protons that are three bonds away. In accordance with the n+1 rule, the signal from the methyl protons is split into three peaks with 1:2:1 relative intensities. The methylene protons appear as a quartet, with the relative intensities of 1:3:3:1.
Qualitatively, any spin plus-half nucleus polarizes the spins of its electrons to the minus-half state. Consequently, the paired electron in the hydrogen–carbon bond must...
1.4K
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

997
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
997

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response to "Comment on 'Effective spin Hamiltonians for the quantum-rotor tunneling problem in pulse EPR'" [J. Chem. Phys. 164, 187101 (2025)].

The Journal of chemical physics·2026
Same author

Topology-Dependent Coke Formation in the Catalytic Pyrolysis of Phenol Over HFAU and HZSM-5 Zeolites.

Angewandte Chemie (International ed. in English)·2026
Same author

Characterization of strongly hyperfine-split protons by DNP.

Physical chemistry chemical physics : PCCP·2026
Same author

Characterization of flexible RNA binding by tandem RNA recognition motifs through integrative ensemble modelling.

Nucleic acids research·2026
Same author

Single atoms of indium on hafnia enable superior CO<sub>2</sub>-based methanol synthesis.

Nature nanotechnology·2026
Same author

Towards full optimisation of automated double electron-electron resonance spectroscopy.

Physical chemistry chemical physics : PCCP·2025

Related Experiment Video

Updated: Dec 31, 2025

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
08:54

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Published on: June 5, 2019

7.9K

Optimal background treatment in dipolar spectroscopy.

Luis Fábregas Ibáñez1, Gunnar Jeschke1

  • 1ETH Zurich, Lab. Phys. Chem., Vladimir-Prelog Weg 2, 8093 Zurich, Switzerland. luis.fabregas@phys.chem.ethz.ch.

Physical Chemistry Chemical Physics : PCCP
|January 7, 2020
PubMed
Summary

This study introduces a novel method for treating background signals in Electron Paramagnetic Resonance (EPR) spectroscopy. The improved approach enhances the accuracy of distance distribution recovery compared to existing techniques.

More Related Videos

Author Spotlight: Using Hyperpolarized Xenon-129 MRI to Study Lung Diseases
09:55

Author Spotlight: Using Hyperpolarized Xenon-129 MRI to Study Lung Diseases

Published on: January 5, 2024

1.7K
Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research
08:12

Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research

Published on: February 16, 2024

14.7K

Related Experiment Videos

Last Updated: Dec 31, 2025

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
08:54

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Published on: June 5, 2019

7.9K
Author Spotlight: Using Hyperpolarized Xenon-129 MRI to Study Lung Diseases
09:55

Author Spotlight: Using Hyperpolarized Xenon-129 MRI to Study Lung Diseases

Published on: January 5, 2024

1.7K
Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research
08:12

Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research

Published on: February 16, 2024

14.7K

Area of Science:

  • Spectroscopy
  • Biophysics
  • Computational Chemistry

Background:

  • Accurate background treatment is crucial for Electron Paramagnetic Resonance (EPR) spectroscopy.
  • Existing methods for background subtraction and division in EPR have limitations.
  • Recovering precise distance distributions relies on effective signal processing.

Purpose of the Study:

  • To identify pitfalls in current background treatment methods for EPR spectroscopy.
  • To propose and validate an improved background treatment approach.
  • To assess the impact of signal truncation and fitting errors on distance distribution accuracy.

Main Methods:

  • Mathematical analysis of background subtraction and division techniques.
  • Development of a novel background treatment algorithm for EPR data.
  • Empirical validation of the proposed method against established techniques.
  • Analysis of post-correction signal truncation and background-fit errors.

Main Results:

  • Identified critical limitations in standard background subtraction and division methods.
  • The proposed improved background treatment method demonstrates superior performance.
  • Established methods can be sensitive to signal truncation and moderate fitting errors.
  • The new approach yields more accurate distance distributions.

Conclusions:

  • The developed background treatment method offers significant improvements for EPR spectroscopy.
  • Accurate background processing is essential for reliable distance distribution analysis.
  • The findings provide valuable insights for optimizing EPR data analysis workflows.