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Published on: October 28, 2021
Development of specific primers for Fusarium oxysporum causing damping off of Lilium formolongi
Takeshi Toda1, Shun Hanesaka2, Kuniaki Shishido3
1Department of Bioproduction Science, Faculty of Bioresource Science, Akita Prefectural University, 241-438 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita, 010-0195, Japan. ttoda@akita-pu.ac.jp.
Abstract:
Primers specific for the hypothetical forma specialis of Fusarium oxysporum were designed to amplify DNA from this pathogenic fungus that infects plants including lilies. The F. oxysporum sequence between the transposal elements han and hop was used for primer design. Three primer pairs designed from this region were confirmed as specific for 24 isolates of F. oxysporum pathogenic to lilies, except for one pathogenic isolates as extraordinary. No amplification was observed from F. oxysporum non-pathogenic to lily, from 12 forma specialis, and 14 fungi and oomycetes concerned with Liliaceae plants. We propose that specific primers designed from this region will be useful to detect isolates of F. oxysporum that are pathogenic to lilies.
Insights
New primers specifically detect Fusarium oxysporum strains pathogenic to lilies. This molecular tool aids in identifying lily-infecting Fusarium oxysporum, crucial for plant disease management.
Area of Science:
- Plant Pathology
- Molecular Biology
- Mycology
Background:
- Fusarium oxysporum is a devastating plant pathogen with numerous formae speciales, including those affecting lilies.
- Accurate identification of pathogenic Fusarium oxysporum isolates is critical for effective disease management and preventing crop loss.
- Existing diagnostic methods may lack the specificity to distinguish between pathogenic and non-pathogenic strains or different formae speciales.
Purpose of the Study:
- To design and validate specific DNA primers for the molecular detection of Fusarium oxysporum forma specialis pathogenic to lilies.
- To assess the specificity of these primers against a range of Fusarium oxysporum isolates and other related fungi.
Main Methods:
- Primer design based on the unique sequence of Fusarium oxysporum between the transposable elements 'han' and 'hop'.
- Polymerase Chain Reaction (PCR) amplification using three primer pairs.
- Testing primer specificity against 24 pathogenic F. oxysporum isolates (lily-specific), one unusual pathogenic isolate, non-pathogenic F. oxysporum, 12 other formae speciales, and 14 other fungi/oomycetes associated with Liliaceae plants.
Main Results:
- Three primer pairs demonstrated high specificity for Fusarium oxysporum isolates pathogenic to lilies.
- One pathogenic isolate showed an unusual reaction, highlighting potential variability.
- No amplification occurred with non-pathogenic F. oxysporum, other formae speciales, or related Liliaceae-associated fungi and oomycetes.
Conclusions:
- The developed primers are effective tools for specifically detecting Fusarium oxysporum isolates pathogenic to lilies.
- This molecular approach offers a reliable method for early and accurate diagnosis of lily Fusarium wilt.
- The findings contribute to improved strategies for managing Fusarium diseases in lily cultivation.
