Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Southern Blot02:57

Southern Blot

22.2K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
22.2K
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

111.5K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
111.5K
Sanger Sequencing01:57

Sanger Sequencing

772.4K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
772.4K
Electrophoresis: Overview01:20

Electrophoresis: Overview

3.4K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wearable-Compatible Detection of Mild Cognitive Impairment Using Novel Features Based on Sleep Stage Dynamics.

Brain sciences·2026
Same author

Development of 2D Microfluidics Surface with Low-Frequency Electric Fields for Cell Separation Applications.

Sensors (Basel, Switzerland)·2025
Same author

A Lightweight Neural Network Based on Memory and Transition Probability for Accurate Real-Time Sleep Stage Classification.

Brain sciences·2025
Same author

The Pre-Polarization and Concentration of Cells near Micro-Electrodes Using AC Electric Fields Enhances the Electrical Cell Lysis in a Sessile Drop.

Biosensors·2025
Same author

Automating the amino acid identification in elliptical dichroism spectrometer with Machine Learning.

PloS one·2025
Same author

A Miniaturized MicroRNA Sensor Identifies Targets Associated with Weight Loss in a Diet and Exercise Intervention among Healthy Overweight Individuals.

Sensors (Basel, Switzerland)·2022
Same journal

Correction: Kang et al. Fluid Flow to Electricity: Capturing Flow-Induced Vibrations with Micro-Electromechanical-System-Based Piezoelectric Energy Harvester. <i>Micromachines</i> 2024, <i>15</i>, 581.

Micromachines·2026
Same journal

Femtosecond Laser Texturing of Wood Coatings with Bio-Based Epoxy and Wax Additives for Enhanced Hydrophobicity.

Micromachines·2026
Same journal

Engineering of Optoelectronic Devices for Renewable Energy Applications.

Micromachines·2026
Same journal

Phase Transformation and Electrochemical Behavior of Hexagonal TiO<sub>2</sub> Nanotubes Under Different Annealing Temperatures and Heating Rates.

Micromachines·2026
Same journal

Process Optimization and Predictive Modeling of Femtosecond Laser Precision Milling for Commercial PMMA Slices.

Micromachines·2026
Same journal

A Hybrid Preprocessing Multi-Objective Surrogate Model for Thermal MEMS Actuators.

Micromachines·2026
See all related articles

Related Experiment Video

Updated: Dec 31, 2025

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.5K

Nucleotide Identification in DNA Using Dielectrophoresis Spectroscopy.

Fleming Dackson Gudagunti1, Logeeshan Velmanickam1, Dharmakeerthi Nawarathna1

  • 1Department of Electrical and Computer Engineering, North Dakota State University, Fargo, ND 58102, USA.

Micromachines
|January 8, 2020
PubMed
Summary
This summary is machine-generated.

Negative dielectrophoresis (DEP) spectroscopy effectively detects single nucleotide polymorphisms (SNPs) in DNA. This biosensor technology analyzes DNA-bound microsphere movement to identify genetic variants linked to diseases.

Keywords:
bioelectronicsdielectrophoresissingle nucleotide polymorphismspectroscopy

More Related Videos

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
04:18

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

Published on: March 31, 2019

14.5K
Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.4K

Related Experiment Videos

Last Updated: Dec 31, 2025

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.5K
DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
04:18

DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

Published on: March 31, 2019

14.5K
Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.4K

Area of Science:

  • Biotechnology
  • Biosensor Technology
  • Molecular Diagnostics

Background:

  • Single nucleotide polymorphisms (SNPs) are key genetic variations associated with various diseases.
  • Accurate and efficient SNP detection is crucial for genetic diagnostics and personalized medicine.
  • Current SNP genotyping methods can be complex and time-consuming.

Purpose of the Study:

  • To demonstrate negative dielectrophoresis (DEP) spectroscopy as a viable biosensor transduction mechanism for SNP detection.
  • To investigate the frequency dependence of DEP forces on DNA-bound microspheres for SNP identification.
  • To assess the potential of this technology for diagnosing genetic variants.

Main Methods:

  • Utilized negative dielectrophoresis (DEP) spectroscopy with interdigitated electrodes.
  • Functionalized polystyrene microspheres (PM) with single-strand DNA.
  • Measured the drift velocity of DEP-bound PMs across a frequency range of 0.5 MHz to 2 MHz.
  • Employed custom automated software with real-time image processing for drift velocity calculation.

Main Results:

  • Observed a clear frequency dependence of the negative DEP force on DNA-bound PMs.
  • Demonstrated that DEP force changes correlate with variations in the last and second-to-last nucleotides of the DNA strand.
  • The drift velocity of PMs, proportional to DEP force, varied measurably with SNP presence.

Conclusions:

  • Negative dielectrophoresis (DEP) spectroscopy is a sensitive and effective method for detecting SNPs in short DNA strands.
  • This biosensor technology offers a promising approach for SNP genotyping.
  • The developed method has significant potential for disease diagnosis and the identification of disease-associated genetic variants.