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Circular RNA circ_001621 promotes osteosarcoma cells proliferation and migration by sponging miR-578 and regulating

Xianglu Ji1, Liping Shan2, Peng Shen1

  • 1Department of Orthopedic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, PR China.

Cell Death & Disease
|January 8, 2020
PubMed

Insights

Circular RNA circ_001621 promotes osteosarcoma progression and metastasis by sponging miR-578, leading to increased vascular endothelial growth factor (VEGF) signaling. This study identifies circ_001621 as a potential therapeutic target for osteosarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma progression is linked to vascular endothelial growth factor (VEGF).
  • Understanding the regulatory mechanisms of osteosarcoma is crucial for developing targeted therapies.
  • Circular RNAs (circRNAs) are emerging as key players in cancer development.

Purpose of the Study:

  • To investigate the role of circ_001621 in osteosarcoma progression.
  • To elucidate the interaction between circ_001621, miR-578, and VEGF in osteosarcoma.
  • To identify novel therapeutic targets for advanced osteosarcoma.

Main Methods:

  • CircRNA microarrays were used to identify upregulated circRNAs.
  • Bioinformatic analysis (Circular RNA Interactome) predicted potential miRNA targets.
  • In vitro and in vivo experiments assessed the functional impact of the circ_001621/miR-578/VEGF axis.
  • Nude mice xenografts were used to evaluate metastasis.

Main Results:

  • Circ_001621 was significantly upregulated in osteosarcoma and correlated with shorter survival.
  • Circ_001621 directly sponges miR-578, leading to the release of its target genes, CDK4 and MMP9.
  • Circ_001621 promotes osteosarcoma proliferation, migration, and metastasis in vitro and in vivo.

Conclusions:

  • Circ_001621 enhances osteosarcoma progression and metastasis by inhibiting miR-578.
  • The circ_001621/miR-578/VEGF pathway represents a novel therapeutic strategy for osteosarcoma.
  • Circ_001621 is a potential biomarker for osteosarcoma prognosis and a therapeutic target.

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