Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Buffers02:56

Buffers

171.8K
A solution containing appreciable amounts of a weak conjugate acid-base pair is called a buffer solution, or a buffer. Buffer solutions resist a change in pH when small amounts of a strong acid or a strong base are added. A solution of acetic acid and sodium acetate is an example of a buffer that consists of a weak acid and its salt: CH3COOH (aq) + CH3COONa (aq). An example of a buffer that consists of a weak base and its salt is a solution of ammonia and ammonium chloride: NH3 (aq) + NH4Cl...
171.8K
Proofreading01:31

Proofreading

8.5K
Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...
8.5K
Proofreading01:43

Proofreading

59.5K
Overview
59.5K
Conserved Binding Sites01:49

Conserved Binding Sites

1.9K
1.9K
Conserved Binding Sites01:49

Conserved Binding Sites

5.0K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
5.0K
Weak Base Solutions03:21

Weak Base Solutions

24.6K
Some compounds produce hydroxide ions when dissolved by chemically reacting with water molecules. In all cases, these compounds react only partially and so are classified as weak bases. These types of compounds are also abundant in nature and important commodities in various technologies. For example, global production of the weak base ammonia is typically well over 100 metric tons annually, being widely used as an agricultural fertilizer, a raw material for chemical synthesis of other...
24.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The role of Smad4 in the regulation of insulin resistance, inflammation and cell proliferation in HTR8-Svneo cells.

Cell biochemistry and function·2020
Same author

An environmental-friendly process for dissociating toxic substances and recovering valuable components from spent carbon cathode.

Journal of hazardous materials·2020
Same author

Clinical observation on therapeutic effect of filiform fire needle for vitiligo: A retrospective study.

Dermatologic therapy·2020
Same author

Decreased expression of prohibitin 1 mediates high<b>-</b>mobility group protein 1<b>-</b>induced pulmonary artery endothelial cell injury.

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences·2020
Same author

Reliability and validity of the Caregiver Reported Early Development Instruments (CREDI) in impoverished regions of China.

BMC pediatrics·2020
Same author

Abnormalities of ILC1 in children with hand, foot and mouth disease during enterovirus 71 infection.

Virology·2020
Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026
Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026
Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026
Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026
Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026
Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026
See all related articles

Related Experiment Video

Updated: Dec 31, 2025

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.9K

Base editors beware.

Lei Tang1

  • 1Nature Methods, . lei.tang@nature.com.

Nature Methods
|January 8, 2020
PubMed
Summary
This summary is machine-generated.

New tools are required to effectively study off-target mutations caused by base editing technologies. This research addresses the need for better methods to analyze unintended genetic alterations from base editors.

More Related Videos

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.5K
Genome Editing with CompoZr Custom Zinc Finger Nucleases ZFNs
09:11

Genome Editing with CompoZr Custom Zinc Finger Nucleases ZFNs

Published on: June 14, 2012

26.0K

Related Experiment Videos

Last Updated: Dec 31, 2025

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.9K
Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.5K
Genome Editing with CompoZr Custom Zinc Finger Nucleases ZFNs
09:11

Genome Editing with CompoZr Custom Zinc Finger Nucleases ZFNs

Published on: June 14, 2012

26.0K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Base editing is a powerful genome editing technology.
  • Off-target mutations are a significant concern with base editors.
  • Accurate detection of these mutations is crucial for safety and efficacy.

Purpose of the Study:

  • To develop and validate novel tools for studying base editor off-target mutations.
  • To provide a reliable method for assessing the specificity of base editing systems.

Main Methods:

  • Development of a new assay for detecting off-target mutations.
  • Application of the assay to evaluate different base editing systems.
  • Bioinformatic analysis of sequencing data to identify mutation profiles.

Main Results:

  • The developed tools successfully identified and quantified off-target mutations.
  • Distinct mutation profiles were observed for different base editors.
  • The sensitivity and specificity of the tools were demonstrated.

Conclusions:

  • The new tools are effective for studying base editor-induced off-target mutations.
  • These tools will aid in the development of safer and more precise base editing applications.
  • Further research can utilize these tools to optimize base editing strategies.