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Updated: Dec 31, 2025
07:36
Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
Published on: May 1, 2015
14.8K
Epigenetic priming by Dot1l in lymphatic endothelial progenitors ensures normal lymphatic development and function
Hyunjin Yoo1, Young Jae Lee2, Chanhyeok Park1
1Department of Stem Cell & Regenerative Biotechnology, Humanized Pig Research Center (SRC), Konkuk University, Seoul, Gwangjin-gu, 05029, Republic of Korea.
Cell Death & Disease
|January 8, 2020
Summary
Dot1l, a histone methyltransferase, is crucial for lymphatic vessel development. Its absence in endothelial cells causes severe lymphatic defects, highlighting its role in epigenetic regulation of lymphatic formation.
Area of Science:
- Vascular Biology
- Epigenetics
- Developmental Biology
Background:
- The lymphatic system is vital for immune function, fluid balance, and lipid absorption.
- Mechanisms regulating lymphatic vessel formation and function are complex, with epigenetic factors poorly understood.
Purpose of the Study:
- To investigate the role of Dot1l, a histone methyltransferase, in the development of lymphatic vessels.
Main Methods:
- Genetic ablation and overexpression of Dot1l in mouse endothelial cells (ECs).
- Analysis of lymphatic vessel morphology and gene expression.
- Focus on Tie2(+) ECs and their role in lymphatic development.
Main Results:
- Loss of Dot1l in Tie2(+) ECs resulted in severe lymphatic anomalies, including edema and blood-lymphatic mixing.
- Dot1l deficiency led to lymphatic aplasia, while overexpression caused hyperplasia.
- Dot1l regulates expression of genes essential for lymphatic endothelial cell (LEC) development.
Conclusions:
- Dot1l is essential for proper lymphatic development through epigenetic regulation of LEC progenitors.
- Dot1l-mediated H3K79 methylation is critical for maintaining lymphatic vessel integrity and function.
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