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Updated: Dec 31, 2025
Detection of Targetable Alterations in Non-small Cell Lung Cancer using Next-generation Sequencing
Published on: October 10, 2025
RNA-Seq Analysis Identified XLOC_009190 as Potential Therapeutic Target for Lung Adenocarcinoma
Jing Wang1, Ning Wang1, Wei-Dong Zhao2
1Department of Surgery, School of Clinical Medicine, Dali University, Dali, Yunnan 671000, People's Republic of China.
Background:
The abnormal regulation on the expression of lncRNAs had been linked to multiple kinds of cancers, including lung adenocarcinoma.
Methods:
In this study, we carried out RNA-Seq on the three tumors and their paired normal samples from Chinese patients with lung adenocarcinoma. All the transcripts were de novo assembled, among which all the possible lncRNAs were predicted by tools including PLEK, CNCI, CPC, Blastp, hmmscan, and so forth. Their expression levels, altogether with the annotated mRNAs, were quantified. The weighted correlation network analysis and analysis of differential expression were carried out to explain the biological function of these novel lncRNAs.
Results:
The weighted correlation network analysis showed that the lncRNAs, which were highly correlated with protein-coding genes, participated in various pathways, including PI3K kinase pathways. These lncRNAs were important regulators in biological processes. Next, the differentially expressed lncRNAs were identified, including four known lncRNAs and one novel lncRNA (XLOC_009190). The cis-regulation of this novel lncRNA might act on MGST1, which protected cells by conjugation and glutathione peroxidase functions. The trans-regulation of this lncRNA was investigated by its correlated mRNAs. The results showed that it possibly played a role in transmembrane receptors like G protein-coupled receptors and potassium channels.
Conclusion:
We proposed the potential biological function of XLOC_009190, but further experiments are needed to elucidate its roles and its potential to be the therapeutic target.
Insights
This study identifies a novel long non-coding RNA (lncRNA), XLOC_009190, in lung adenocarcinoma. It may regulate cellular protection and transmembrane receptor pathways, offering potential therapeutic targets.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Aberrant long non-coding RNA (lncRNA) expression is implicated in various cancers, including lung adenocarcinoma.
- lncRNAs play crucial roles in tumorigenesis and cancer progression.
Purpose of the Study:
- To identify and characterize novel lncRNAs in lung adenocarcinoma.
- To investigate the potential biological functions and regulatory roles of these lncRNAs.
Main Methods:
- RNA-Sequencing (RNA-Seq) was performed on lung adenocarcinoma tumors and paired normal samples.
- lncRNAs were predicted using bioinformatics tools and their expression quantified.
- Weighted correlation network analysis and differential expression analysis were employed.
Main Results:
- lncRNAs correlated with protein-coding genes were found to be involved in pathways like PI3K signaling.
- A novel lncRNA, XLOC_009190, was identified alongside four known lncRNAs.
- XLOC_009190 showed potential cis-regulation of MGST1 and trans-regulation involving G protein-coupled receptors and potassium channels.
Conclusions:
- The study proposes the potential functions of the novel lncRNA XLOC_009190 in lung adenocarcinoma.
- Further research is required to fully elucidate the roles of XLOC_009190 and its therapeutic potential.
