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Updated: Dec 31, 2025
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Mouse Models Of Helicobacter Infection And Gastric Pathologies
Published on: October 18, 2018
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Improved method for inducing chronic atrophic gastritis in mice
Xian Wei1, Xue-Ping Feng1, Lu-Yao Wang1
1Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China.
World Journal of Gastrointestinal Oncology
|January 8, 2020
Summary
Developing a mouse model for chronic atrophic gastritis (CAG) is crucial for understanding gastric cancer. A combined approach using Helicobacter pylori, MNNG, and ammonia effectively created a stable CAG mouse model.
Area of Science:
- Gastroenterology and Hepatology
- Oncology
- Animal Models
Background:
- Chronic atrophic gastritis (CAG) is a precancerous condition for gastric cancer.
- Gastric cancer pathogenesis is unclear, necessitating research into prevention and treatment.
- Developing a stable animal model is essential for studying CAG reversal and gastric cancer prevention.
Purpose of the Study:
- To establish a rapid, stable, and safe mouse model for human chronic atrophic gastritis (CAG).
Main Methods:
- Kunming mice were divided into control, Helicobacter pylori (H. pylori), N-methyl-N'-nitroguanidine (MNNG), ammonia water, and a combined H. pylori, MNNG, and ammonia group.
- CAG model establishment was assessed via gastric mucosa staining (hematoxylin eosin, terminal nick-end labelling, immunohistochemistry for Bax and Bcl-2).
- H. pylori colonization, inflammatory factors (IL-1β, IL-8, TNF-α), and immune markers (CD4, CD8) were analyzed.
Main Results:
- The combined group exhibited slight CAG at 90 days and moderate CAG at 120 days.
- Other experimental groups did not show signs of CAG within the study period.
- The combined method demonstrated efficacy in inducing CAG in the mouse model.
Conclusions:
- A combination of H. pylori, MNNG, and ammonia provides an effective method for developing a mouse model of human CAG.
- This model can facilitate further research into CAG pathogenesis, prevention, and treatment strategies.
- The established model supports the investigation of reversing precancerous lesions to reduce gastric cancer incidence.
