RNA sequencing data of mouse 2-cell embryos treated with DMSO
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Updated: Dec 31, 2025
Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
Published on: January 7, 2020
Min-Hee Kang1, Seong-Yeob You1, Kwonho Hong1
1Department of Stem Cell and Regenerative Biotechnology, Humanized Pig Research Center (SRC), Konkuk University, Seoul, Republic of Korea.
Abstract:
To understand the effect of DMSO in preimplantation embryos, we have treated mouse 1 cell zygotes with DMSO and found that DMSO treatment caused 2 or 4 cell embryonic arrest and altered the acetylation levels of mouse preimplantation embryos To illustrate the mechanism of DMSO in mouse preimplantation embryos, fertilized zygotes have been treated with 2% of DMSO and then performed RNA-seq analyses. Differentially expressed genes were identified using DESeq2 after adjustment for false discovery rate (FDR q value < 0.05). Gene Set Enrichment Analysis (GSEA) was also performed to identify biological pathways significantly modulated by DMSO. Raw and processed RNA-seq data were deposited and made publicly available on the Gene Expression Omnibus (GEO; GSE124598). The data presented in this article are related to the research paper entitled "DMSO impairs the transcriptional program for maternal-to-embryonic transition by altering histone acetylation", available in Biomaterials [1].
Insights
Dimethyl sulfoxide (DMSO) causes developmental arrest in early mouse embryos by altering gene expression and histone acetylation. This study reveals DMSO
Area of Science:
- Developmental Biology
- Epigenetics
- Toxicology
Background:
- Dimethyl sulfoxide (DMSO) is widely used in biological research and cryopreservation.
- Its effects on early embryonic development, particularly at the molecular level, require further elucidation.
- Understanding DMSO's impact is crucial for optimizing assisted reproductive technologies and interpreting experimental results.
Purpose of the Study:
- To investigate the impact of DMSO on mouse preimplantation embryonic development.
- To elucidate the molecular mechanisms underlying DMSO-induced developmental effects, focusing on gene expression and histone acetylation.
- To provide publicly accessible data for further research.
Main Methods:
- Mouse zygotes were treated with DMSO (2%).
- Embryonic development was monitored for cell cycle arrest (2-cell and 4-cell stages).
- RNA sequencing (RNA-seq) and Gene Set Enrichment Analysis (GSEA) were performed to identify differentially expressed genes and affected pathways.
Main Results:
- DMSO treatment resulted in significant 2-cell and 4-cell embryonic arrest in mouse zygotes.
- Altered histone acetylation levels were observed in DMSO-treated embryos.
- RNA-seq analysis identified differentially expressed genes and modulated biological pathways, indicating disruption of the maternal-to-embryonic transition transcriptional program.
Conclusions:
- DMSO impairs mouse preimplantation embryonic development by inducing cell cycle arrest.
- DMSO alters the epigenetic landscape, specifically histone acetylation, in early embryos.
- The findings highlight DMSO's potential to disrupt critical developmental processes, including the maternal-to-embryonic transition.
