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Updated: Dec 31, 2025
A Model for Epilepsy of Infectious Etiology using Theiler's Murine Encephalomyelitis Virus
Published on: June 23, 2022
Proteome dataset of mouse macrophage cell line infected with tick-borne encephalitis virus
A L Rusanov1, A A Stepanov1, V G Zgoda1
1V. N. Orehovich Institute of Biomedical Chemistry, Russian Federation.
Abstract:
We report the proteomic datasets on the mouse macrophage cell line PMJ2R infected with tick-borne encephalitis virus (TBEV) for two and six days. Data were acquired using shotgun ultra-high resolution mass spectrometry. Peptide identifications were done using the Mascot version 2.4 (Matrix Science), and quantification was performed by a label-free approach. Protein profiles of early (two days) and late (six days) stages of infection were compared between each other and the respective control samples. Protein profiles of infected and control samples differed in the number of identified proteins and their relative abundances. Proteins detected in the TBEV-infected cells were involved in various processes related to the infection, including defense response against the virus, regulation of viral process, negative regulation of viral genome replication, RNA binding, or innate immune response. Also, proteins specific for the early and late stages of infection were identified.
Insights
This study reveals proteomic changes in mouse macrophages infected with tick-borne encephalitis virus (TBEV). Key immune and viral response proteins were identified at early and late infection stages.
Area of Science:
- Virology
- Immunology
- Proteomics
Background:
- Tick-borne encephalitis virus (TBEV) poses a significant threat to public health.
- Understanding host-pathogen interactions at the molecular level is crucial for developing effective countermeasures.
- Macrophage responses are central to the innate immune defense against viral infections.
Purpose of the Study:
- To investigate the proteomic alterations in mouse macrophages upon TBEV infection.
- To compare protein profiles at different stages of TBEV infection (2 and 6 days post-infection).
- To identify host proteins involved in antiviral defense and viral processes.
Main Methods:
- Shotgun ultra-high resolution mass spectrometry was employed for data acquisition.
- Peptide identification was performed using Mascot version 2.4.
- Label-free quantification was utilized to compare protein abundances.
Main Results:
- Significant differences in protein profiles and abundances were observed between TBEV-infected and control macrophages.
- Proteins involved in defense response, viral process regulation, and innate immunity were upregulated in infected cells.
- Distinct protein sets were identified specific to early (2 days) and late (6 days) stages of infection.
Conclusions:
- TBEV infection induces substantial proteomic changes in macrophages, impacting key cellular processes.
- The identified proteins provide insights into the host's antiviral mechanisms and viral evasion strategies.
- This dataset serves as a valuable resource for further research into TBEV pathogenesis and host-pathogen interactions.
