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Patterns of spon1b:GFP expression during early zebrafish brain development.

Nathalie Agudelo-Dueñas1,2, Manu Forero-Shelton1, Irina V Zhdanova3

  • 1Biophysics Group, Department of Physics, Universidad de los Andes, Bogotá, Colombia.

BMC Research Notes
|January 9, 2020
PubMed
Summary

F-spondin (spon1b) expression in developing zebrafish brains was mapped using Tg(spon1b:GFP) transgenic lines. Light sheet fluorescence microscopy revealed spon1b:GFP in sensory-motor modulating brain regions, offering insights into early neural development.

Keywords:
F-spondin/spon1bGFPHabenulaLight sheet fluorescence microscopy (LSFM)Olfactory systemPituitary/hypophysisZebrafish (Danio rerio)

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Extracellular Matrix Research

Background:

  • F-spondin is an evolutionarily conserved extracellular matrix protein crucial for neuronal development.
  • Previous studies characterized F-spondin expression in adult zebrafish brains.
  • Its role in embryonic development, particularly in the floor plate promoting neuronal outgrowth, is known.

Purpose of the Study:

  • To detail green fluorescent protein (GFP) expression driven by the spon1b promoter in the developing zebrafish brain.
  • To utilize the transgenic Tg(spon1b:GFP) zebrafish line for in vivo imaging.
  • To employ light sheet fluorescence microscopy (LSFM) for high-resolution visualization.

Main Methods:

  • Generation and use of the transgenic Tg(spon1b:GFP) zebrafish line.
  • Light Sheet Fluorescence Microscopy (LSFM) for live embryo imaging.
  • Observation of GFP expression patterns from 22 to 96 hours post fertilization.

Main Results:

  • Confirmed spon1b:GFP expression in the telencephalon and diencephalon (olfactory bulbs, habenula, optic tectum, medial longitudinal fasciculus nuclei).
  • Identified novel spon1b:GFP expression in the pituitary anlage, dorso-rostral cluster, and ventro-rostral cluster.
  • LSFM enabled tracking of dynamic cellular migration patterns during development.

Conclusions:

  • Larval spon1b:GFP expression initiates early in specific brain structures involved in sensory-motor modulation.
  • LSFM imaging of the Tg(spon1b:GFP) line is an effective method for in vivo characterization of gene expression patterns.
  • This study provides a detailed map of F-spondin expression during zebrafish brain development.