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Updated: Dec 31, 2025
08:23
CIRCLE-Seq for Interrogation of Off-Target Gene Editing
Published on: November 1, 2024
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Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing
Jinjing Li1, Shunyan Hong1, Wanjin Chen1
1Department of Neurology and Institute of Neurology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China; Fujian Key Laboratory of Molecular Neurology, Fujian Medical University, Fuzhou, 350005, China.
Journal of Genetics and Genomics = Yi Chuan Xue Bao
|January 9, 2020
Summary
CRISPR gene editing offers powerful genome manipulation but faces efficiency and off-target challenges. This review covers CRISPR versions and detection methods, highlighting future research directions for improved gene editing.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- CRISPR-Cas systems are revolutionary genome editing tools.
- Current limitations include poor efficiency and off-target effects.
- Advanced CRISPR versions improve on-target efficiency, but off-target detection remains difficult.
Purpose of the Study:
- To review different CRISPR systems and their advanced versions.
- To outline various off-target detection strategies.
- To discuss the implications for future gene editing research.
Main Methods:
- Literature review of CRISPR technologies.
- Analysis of different CRISPR-Cas systems and base editors.
- Evaluation of off-target detection methods.
Main Results:
- CRISPR efficiency has improved, but off-target detection is a significant challenge.
- Various off-target detection strategies have different merits and limitations.
- Further research is needed to refine detection methods.
Conclusions:
- Addressing off-target effects is crucial for safe and effective CRISPR applications.
- Improved detection methods will advance the field of genome editing.
- This review provides a foundation for future gene editing research.
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