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Dynamic spatiotemporal expression pattern of limbal stem cell putative biomarkers during mouse development.
Zhi Hou Guo1, Yi Ming Zeng2, Jun Sheng Lin3
1School of Medicine, Huaqiao University, Quanzhou, 362021, Fujian, China; Stem Cell Laboratory, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, China.
Experimental Eye Research
|January 9, 2020
Summary
Identifying limbal stem cell (LSC) biomarkers in mice is crucial for corneal health. This study reveals age-related expression patterns of LSC markers, identifying CK15 as a reliable biomarker after postnatal day 14.
Area of Science:
- Ophthalmology
- Developmental Biology
- Stem Cell Biology
Background:
- Limbal stem cells (LSCs) are vital for corneal epithelium maintenance and repair.
- Identifying specific LSC biomarkers remains a significant challenge.
- Mouse models offer controllable sampling for studying LSC development.
Purpose of the Study:
- To investigate the spatiotemporal expression patterns of putative LSC biomarkers during mouse development.
- To identify reliable biomarkers for mouse LSCs, particularly in postnatal stages.
Main Methods:
- Morphological analysis of pre- and post-natal mouse limbus and cornea.
- Immunohistochemistry (IHC) staining for LSC putative biomarkers (Vim, p63, CK14, CK15).
- Analysis of biomarker expression dynamics relative to developmental age.
Main Results:
- The Palisades of Vogt (POV) are absent in mice.
- CECs undergo rapid stratification after eyelid opening (P14).
- Biomarker expression order in limbal basal cells: Vim = p63 > CK14 > CK15.
- Biomarker expression order in corneal basal cells: Vim > p63 > CK15 > CK14.
- CK15 expression is undetectable in CECs after P14, suggesting its suitability as a specific postnatal LSC biomarker.
Conclusions:
- Mouse LSC biomarker expression is age-dependent.
- CK15 is a suitable biomarker for mouse LSCs after P14.
- Understanding dynamic biomarker expression aids in comprehending stemness maintenance.
