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Updated: Dec 31, 2025
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Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids
Published on: October 21, 2022
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Mitochondrial Ca2+ laMety directs fibrosis
Malina Doynova1, Konstantinos Chatzieleftheriadis1, H Llewelyn Roderick2
1KU Leuven, Department of Cardiovascular Sciences, Laboratory of Experimental Cardiology, 3000 Leuven, Belgium.
Cell Calcium
|January 9, 2020
Summary
Reduced mitochondrial calcium uptake triggers metabolic changes, altering the epigenome and gene expression. This process drives fibroblast to myofibroblast differentiation, impacting cellular remodeling during development and disease.
Area of Science:
- Cellular Biology
- Metabolic Regulation
- Epigenetics
Background:
- Cellular metabolism undergoes significant remodeling during development, disease, or environmental changes.
- Mitochondrial calcium (Ca2+) uptake is a key regulator of cellular processes.
- Recent findings link reduced mitochondrial Ca2+ uptake to metabolic remodeling.
Purpose of the Study:
- To investigate the downstream effects of reduced mitochondrial Ca2+ uptake on cellular metabolism and differentiation.
- To elucidate the role of epigenomic alterations in mediating metabolic changes.
- To understand the link between metabolic remodeling and fibroblast to myofibroblast differentiation.
Main Methods:
- Analysis of cellular metabolic pathways.
- Epigenomic profiling to assess changes in gene regulation.
- Gene expression analysis to identify specific markers.
- Cellular differentiation assays.
Main Results:
- Reduced mitochondrial Ca2+ uptake was confirmed to induce significant metabolic remodeling.
- Metabolic alterations were found to stimulate changes in the epigenome.
- These epigenomic changes led to altered gene expression patterns.
- The observed changes were associated with fibroblast to myofibroblast differentiation.
Conclusions:
- Reduced mitochondrial Ca2+ uptake is a critical trigger for metabolic and epigenomic reprogramming.
- This reprogramming directly influences fibroblast to myofibroblast differentiation.
- Understanding this pathway offers insights into cellular plasticity in development and disease.
Keywords:
Calcium signallingEpigenomeFibrosisMetabolic remodellingMitochondrial calciumMyofibroblastsMore Related Videos
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