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Related Concept Videos

Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

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Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
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The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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Tooth Anatomy01:21

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The human tooth enables us to eat a variety of foods, speak clearly, and even aid in shaping our faces. Teeth are composed of various elements that work together. Here's a detailed look at the anatomy of a human tooth.
The Crown, Neck, and Root
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Determination01:51

Determination

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During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
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Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

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Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
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Updated: Dec 31, 2025

Isolation of Epithelial Cells from Human Dental Follicle
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Epithelial Wnt10a Is Essential for Tooth Root Furcation Morphogenesis.

M Yu1, Y Liu1, Y Wang1

  • 1Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing, China.

Journal of Dental Research
|January 9, 2020
PubMed
Summary
This summary is machine-generated.

Epithelial WNT10A (Wingless-type MMTV integration site family, member 10A) is essential for tooth root furcation formation. Its absence causes taurodontism-like defects by altering WNT4 expression and mesenchymal cell proliferation.

Keywords:
Wnt signalingcell proliferationdevelopmenttaurodontismtooth abnormalitiestooth root

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Area of Science:

  • Developmental Biology
  • Genetics
  • Oral Biology

Background:

  • WNT10A is crucial for tooth development, and its mutation causes taurodontism.
  • The specific role of epithelial versus mesenchymal WNT10A in root furcation formation is unclear.

Purpose of the Study:

  • To investigate the role of WNT10A in tooth root development.
  • To clarify the function of epithelial WNT10A in root furcation morphogenesis.

Main Methods:

  • Generated tissue-specific WNT10A conditional knockout mice (whole tissue and dental epithelium).
  • Utilized RNAscope, immunofluorescent staining, EdU incorporation, RNA-seq, shRNA adenovirus, and kidney capsule grafts.
  • Analyzed cell proliferation and gene expression patterns during root development.

Main Results:

  • Epithelial WNT10A knockout led to absent or apical root furcation, resembling taurodontism.
  • Decreased epithelial cell proliferation and increased pulpal mesenchymal cell proliferation were observed.
  • Epithelial WNT10A knockout upregulated WNT4 and AXIN2 expression in the dental papilla.
  • Suppression of elevated WNT4 partially rescued the root furcation defect.

Conclusions:

  • Epithelial WNT10A is essential for guiding root furcation formation.
  • WNT10A regulates mesenchymal cell proliferation and WNT4 expression during root furcation morphogenesis.