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Isolation of Epithelial Cells from Human Dental Follicle
Published on: November 5, 2021
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Epithelial Wnt10a Is Essential for Tooth Root Furcation Morphogenesis.
1Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing, China.
Journal of Dental Research
|January 9, 2020
Summary
Epithelial WNT10A (Wingless-type MMTV integration site family, member 10A) is essential for tooth root furcation formation. Its absence causes taurodontism-like defects by altering WNT4 expression and mesenchymal cell proliferation.
Area of Science:
- Developmental Biology
- Genetics
- Oral Biology
Background:
- WNT10A is crucial for tooth development, and its mutation causes taurodontism.
- The specific role of epithelial versus mesenchymal WNT10A in root furcation formation is unclear.
Purpose of the Study:
- To investigate the role of WNT10A in tooth root development.
- To clarify the function of epithelial WNT10A in root furcation morphogenesis.
Main Methods:
- Generated tissue-specific WNT10A conditional knockout mice (whole tissue and dental epithelium).
- Utilized RNAscope, immunofluorescent staining, EdU incorporation, RNA-seq, shRNA adenovirus, and kidney capsule grafts.
- Analyzed cell proliferation and gene expression patterns during root development.
Main Results:
- Epithelial WNT10A knockout led to absent or apical root furcation, resembling taurodontism.
- Decreased epithelial cell proliferation and increased pulpal mesenchymal cell proliferation were observed.
- Epithelial WNT10A knockout upregulated WNT4 and AXIN2 expression in the dental papilla.
- Suppression of elevated WNT4 partially rescued the root furcation defect.
Conclusions:
- Epithelial WNT10A is essential for guiding root furcation formation.
- WNT10A regulates mesenchymal cell proliferation and WNT4 expression during root furcation morphogenesis.
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