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Conventional and unconventional interactions of the transcription factor FOXL2 uncovered by a proteome-wide analysis

May Penrad-Mobayed1, Caroline Perrin1, Laetitia Herman1

  • 1Institut Jacques Monod, CNRS UMR7592, Université de Paris, Paris, France.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|January 10, 2020
PubMed

Insights

Researchers identified 255 protein partners of the transcription factor FOXL2, revealing its unexpected roles in mRNA processing, chromatin remodeling, and DNA repair beyond its known functions in ovarian development.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Transcription factors (TFs) regulate gene expression.
  • Understanding TF interactors is key to elucidating their cellular functions.
  • FOXL2 is a TF crucial for ovarian development and implicated in various cellular processes.

Purpose of the Study:

  • To identify endogenous protein partners of FOXL2.
  • To explore the diverse cellular roles of FOXL2 through its interactome.

Main Methods:

  • Proteome-wide analysis using co-immunoprecipitation.
  • Utilized murine granulosa cell-derived AT29c and pituitary-derived alpha-T3 cell lines.
  • Mass spectrometry was employed for data analysis and stringent selection.

Main Results:

  • Identified a core set of 255 FOXL2 protein partners common to both cell lines.
  • Co-precipitated complexes involved in mRNA processing, chromatin remodeling, and DNA replication/repair.
  • Validated interactions, suggesting novel roles for FOXL2.

Conclusions:

  • FOXL2 interacts with a broad range of proteins involved in fundamental cellular processes.
  • FOXL2 plays unexpected roles in mRNA processing, chromatin remodeling, and DNA repair.
  • This study provides a comprehensive view of the FOXL2 interactome and its diverse functions.
Keywords:
DNA recombination and repairRNA processingmRNA splicingprotein‐protein interactionproteomics

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