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Regulated mRNA Transport

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...

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Updated: Dec 31, 2025

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Polylactide-Based Reactive Micelles as a Robust Platform for mRNA Delivery.

Céline Lacroix¹, Almudena Humanes¹, Céline Coiffier¹

¹Biologie Tissulaire et Ingénierie Thérapeutique, IBCP, Université Lyon 1, CNRS, UMR 5305, 69367, Lyon, France.

Pharmaceutical Research

|January 10, 2020

Summary

This study developed poly(D,L-lactide) (PLA)-based micelles for messenger RNA (mRNA) delivery. The novel micellar carriers effectively protected mRNA from degradation and showed improved transfection in dendritic cells compared to peptide-mRNA complexes.

Keywords:

Micelles cationic fusogenic peptide mRNA delivery polylactide

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Area of Science:

Biomaterials Science
Nanotechnology
Drug Delivery Systems

Background:

Messenger RNA (mRNA) therapeutics offer significant potential but face challenges in stability and endosomal escape.
Effective delivery systems are crucial for mRNA's therapeutic applications.

Purpose of the Study:

To design and evaluate poly(D,L-lactide) (PLA)-based micelles for enhanced mRNA delivery.
To overcome mRNA instability and endosomal entrapment using a novel micelle platform.

Main Methods:

Two strategies, layer-by-layer (LbL) and peptide-mRNA pre-complex (PC), were used to immobilize mRNA onto PLA micelles.
Characterization included size, charge, mRNA protection, serum stability, cytotoxicity, and transfection efficiency in dendritic cells (DCs).

Main Results:

Micellar assemblies provided complete mRNA protection in serum, unlike peptide-mRNA complexes which led to degradation.
PLA micelles significantly reduced toxicity compared to peptide-mRNA complexes.
The LbL approach demonstrated superior transfection efficiency in hard-to-transfect DCs.

Conclusions:

The developed PLA/peptide micelle platform is a promising system for improving mRNA stability and delivery.
This platform offers potential for further functionalization via PLA core encapsulation.