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Bioinspired pH-Sensitive Fluorescent Peptidyl Nanoparticles for Cell Imaging.

Jia Kong1, Jiaxing Zhang1, Yuefei Wang1,2

  • 1State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology , Tianjin University , Tianjin 300072 , P. R. China.

ACS Applied Materials & Interfaces
|January 10, 2020
PubMed
Summary

Researchers developed a novel tyrosine-based peptide that exhibits green luminescence, mimicking green fluorescent proteins (GFPs). This peptide is cell-permeable and suitable for stable biomedical imaging applications.

Keywords:
cell imagingfluorescencegreen fluorescent proteinself-assemblytyrosine

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Area of Science:

  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Background:

  • Green fluorescent proteins (GFPs) are essential tools in biomedical research for visualizing cells and biomolecules.
  • Developing novel fluorescent agents with improved properties is crucial for advancing biological imaging.

Purpose of the Study:

  • To construct a novel tyrosine-based peptide with green luminescence inspired by GFPs.
  • To evaluate the peptide's properties for cell imaging applications.

Main Methods:

  • A tyrosine-based peptide was synthesized, inspired by the chromophore of GFPs.
  • The peptide's luminescence, aggregation state, and stabilization via intermolecular hydrogen bonding were analyzed.
  • Cell permeability and endosome escape were achieved by conjugating the peptide with the GPGR motif.
  • Photostability, pH-sensitivity, and biocompatibility were assessed under physiological conditions.

Main Results:

  • The synthesized tyrosine-based peptide demonstrated green luminescence in its aggregated state.
  • The peptidyl nanoparticles were stabilized by intermolecular hydrogen bonding, similar to GFPs.
  • The peptide exhibited cell permeability and endosome escape when conjugated with the GPGR motif.
  • The peptide showed excellent photostability, pH-sensitivity, and biocompatibility, suitable for stable cell imaging.

Conclusions:

  • A novel tyrosine-based peptide with GFP-like green luminescence was successfully constructed.
  • The peptide's properties make it a promising candidate for cell-permeable and stable biomedical imaging.
  • This work offers a new strategy for developing peptidyl bioluminescent agents for diverse biomedical applications.