Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Plasmids01:28

Plasmids

946
Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
946
Antibiotic Selection00:57

Antibiotic Selection

59.2K
Overview
59.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

Thymidylate synthase inhibitory drugs induce p53-dependent pathways differently.

PloS one·2026
Same journal

Top-down and bottom-up attention for joint pattern classification and reconstruction.

PloS one·2026
Same journal

Short- and long-term scaling behavior of blood pressure and pulse arrival time during sleep in healthy controls and patients with obstructive sleep apnea.

PloS one·2026
Same journal

Double DQN-based secrecy energy efficiency and fairness performance in IRS-assisted NOMA systems with friendly jamming.

PloS one·2026
Same journal

10 recommendations for strengthening citizen science for improved societal and ecological outcomes: A co-produced analysis of challenges and opportunities in the 21st century.

PloS one·2026
Same journal

Paying in public: Peer effects, impression management, and willingness to pay on digital payment platforms.

PloS one·2026
See all related articles

Related Experiment Video

Updated: Dec 31, 2025

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.8K

Repository-based plasmid design.

Joshua J Timmons1, Doug Densmore1,2,3

  • 1Lattice Automation Inc., Boston, Massachusetts, United States of America.

Plos One
|January 10, 2020
PubMed
Summary
This summary is machine-generated.

A new software tool efficiently designs plasmids by finding cost-effective DNA fragments in public repositories, reducing costs by 34% compared to fully synthetic methods. This improves plasmid assembly workflows.

More Related Videos

Use of In Vivo Assembly for High-efficiency Plasmid Construction
06:25

Use of In Vivo Assembly for High-efficiency Plasmid Construction

Published on: February 7, 2025

1.3K
Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

14.5K

Related Experiment Videos

Last Updated: Dec 31, 2025

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.8K
Use of In Vivo Assembly for High-efficiency Plasmid Construction
06:25

Use of In Vivo Assembly for High-efficiency Plasmid Construction

Published on: February 7, 2025

1.3K
Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

14.5K

Area of Science:

  • Synthetic biology
  • Bioinformatics
  • Molecular biology

Background:

  • The past decade has seen a dramatic increase in commercially available DNA sequences in repositories like iGEM, Addgene, and DNASU, with plasmid numbers rising from 12,000 to over 300,000.
  • Effectively utilizing these vast repository resources for biodesign remains a significant challenge.

Purpose of the Study:

  • To develop and characterize a software application for optimized plasmid design.
  • To enable the seamless, cost-effective, and accurate assembly of plasmids using existing DNA sequences from repositories.

Main Methods:

  • The software designs plasmids by identifying the most economical combination of synthetic and PCR-amplified repository DNA fragments for Gibson assembly®.
  • It searches user-specified and public DNA databases (iGEM, Addgene, DNASU) for suitable sequences.
  • The approach was validated against iGEM composite parts (post-2005) and Addgene vectors (2018).

Main Results:

  • The software reduces plasmid design costs by 34% compared to a purely synthetic DNA approach.
  • It effectively identifies and integrates existing DNA sequences from major repositories into new plasmid designs.

Conclusions:

  • The developed software significantly improves plasmid assembly workflows.
  • It offers benefits in terms of reduced design time, enhanced build quality, and lower overall costs for biodesign projects.