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Updated: Dec 31, 2025
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
Published on: January 3, 2020
Differences in cellular damage induced by dielectric barrier discharge plasma between Salmonella Typhimurium and
Mingming Huang1, Hong Zhuang2, Jianying Zhao3
1College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, PR China; National Center of Meat Quality & Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Abstract:
Dielectric barrier discharge (DBD) plasma treatments are more effective against Gram negative bacteria than Gram-positive bacteria. Effects of DBD plasma were compared on Salmonella Typhimurium and Staphylococcus aureus cells. Physical cell membrane integrity and function, deoxyribonucleic acid (DNA) oxidation, and intracellular reactive oxygen species (ROS) were measured. The difference in physical damage caused by plasma on Gram-negative and Gram positive bacteria implied a difference in cellular damage pattern, which may be due to differences in cell structure and composition. Antimicrobial particles in plasma react directly with the outer membranes of Gram-negative bacteria and eventually kill them; however, ROS produced from plasma first penetrated the cell wall and membrane of Gram-positive bacteria before reacting with internal cellular components and killing the bacteria.
