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Updated: Dec 31, 2025
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
Published on: March 2, 2017
Development and Evaluation of Modified Cryopreservation for Long-Term Storage of Blastocystis Subtypes 1-3 and 6
Seyed Ahmad Karamati1, Hamid Asadzadeh Aghdaei2, Maryam Niyyati3
1Department of Medical Parasitology and Mycology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Background:
Blastocystis is a protozoan parasite living in the intestine of humans and a wide range of animals. Although Blastocystis grows in several cultivation media, axenification and serial cultivations for long time are the main challenges of the researchers. Therefore, the long-term storage of subtypes/strains of Blastocystis using cryopreservation provides a suitable source of this parasite for the physiological, biochemical, and biological studies.
Methods:
In the current study, seven xenic isolates including two separated isolates from ST1-3 and one isolate from ST6 were cryopreserved using a standard method with minor modifications. After 3 months, all isolates were recovered and cultivated in DMEM medium.
Results:
The findings of the method showed all seven isolates were successfully recovered in DMEM medium. In addition, all isolates remained viable after several sub-cultures.
Conclusions:
It seems that cryopreservation is a simple method that can provide a suitable condition for the long-term storage of Blastocystis.
