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Updated: Dec 31, 2025

Polygraphic Recording Procedure for Measuring Sleep in Mice
Published on: January 25, 2016
Tanacetum parthenium enhances pentobarbital-induced sleeping behaviors
Fatemeh Forouzanfar1,2, Hamed Ghazavi3, Mohammad Mahdi Vahedi4,5
1Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Objective:
Sleep disorders are among the most common psychiatric and medical conditions. In the present study, the hypnotic effect of Tanacetum parthenium was studied in mice.
Materials And Methods:
The hydro-alcoholic extract (HAE) of T. parthenium and three fractions of it, namely water fraction (WF), ethyl acetate fraction (EAF), and n-hexane fraction (NHF), were intraperitoneally (ip) administrated to mice 30 min before injection of sodium pentobarbital (30 mg/kg, ip). Then, 30 min after administration of HAE, motor coordination (rota-rod test) was evaluated. Besides, LD50 of HAE was determined and the cytotoxicity of HAE was evaluated in PC12 cells using the MTT assay.
Results:
HAE 50-200 mg/kg increased the sleeping time. EAF was the only fraction which could prolong the sleep duration and decrease sleep latency. The LD50 value was 4.8 g/kg. The extract induced no cytotoxic effects in PC12 cell line.
Conclusion:
The results suggested that T. parthenium potentiates pentobarbital hypnosis without causing toxic effects. Probably, its effects are mediated by the components present in EAF of this plant.
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